Assembly of keratin onto PtK1 cytoskeletons: evidence for an intermediate filament organizing center. J Cell Biol 1982 Feb;92(2):575-8
Date
02/01/1982Pubmed ID
6174532Pubmed Central ID
PMC2112089DOI
10.1083/jcb.92.2.575Scopus ID
2-s2.0-0020037986 (requires institutional sign-in at Scopus site) 44 CitationsAbstract
Purified keratin, solubilized in 8 M of urea, was added to Triton X-100-extracted PtK1 cells in 5 mM PIPES buffer. The buffer conditions induced assembly of keratin filaments which appear to associate with nuclei of extracted cells. These keratin fibers extend beyond the original margin of the cells and frequently form bridges between adjacent cells. Electron microscopy shows that keratin filaments associate closely with the surface of the nucleus. We suggest that the site of association between keratin and the nucleus may represent an intermediate filament organizing center.
Author List
Eckert BS, Daley RA, Parysek LMMESH terms used to index this publication - Major topics in bold
AnimalsAntigen-Antibody Reactions
Cells, Cultured
Cytoplasm
Cytoskeleton
Keratins
