Faculty Collaboration Database

Decreased NKCC1 activity in erythrocytes from African Americans with hypertension and dyslipidemia. Am J Hypertens 2010 Mar;23(3):321-6

Date

01/02/2010

Pubmed ID

20044742

Pubmed Central ID

PMC3727424

DOI

10.1038/ajh.2009.249

Scopus ID

2-s2.0-76749116178 (requires institutional sign-in at Scopus site)   11 Citations

Abstract

BACKGROUND: Recent studies demonstrated a key role of ubiquitous isoform of Na+,K+,2Cl- co-transport (NKCC1) in regulation of myogenic tone and peripheral resistance. We examined the impact of race, gender, and plasma lipid on NKCC1 activity in French Canadians and African Americans with hypertension and dyslipidemia.

METHODS: NKCC and passive erythrocyte membrane permeability to K+, measured as ouabain-resistant, bumetanide-sensitive, and (ouabain+bumetanide)-resistant 86Rb influx, respectively, were compared in 111 French-Canadian men, 107 French-Canadian women, 26 African-American men, and 45 African-American women with essential hypertension and dyslipidemia.

RESULTS: The African-American men and women were 7 years younger and presented twofold decreased plasma triglycerides compared to their French-Canadian counterparts (P < 0.01) whereas body mass index (BMI), total cholesterol, low-density lipoprotein, and high-density lipoprotein (HDL) were not different. NKCC was respectively 50 and 38% lower in the African-American men and women than in the French Canadians (P < 0.005) without any differences in passive erythrocyte membrane permeability for K+. We did not observe any impact of age on NKCC in all groups under investigation, whereas plasma triglycerides correlated positively with the activity of this carrier in the French-Canadian men only.

CONCLUSIONS: NKCC1 activity is lower in erythrocytes of African Americans with essential hypertension and dyslipidemia than in Caucasian counterparts. We suggest that decreased NKCC1 may contribute to the feature of the pathogenesis of salt-sensitive hypertension seen in African Americans.

Author List

Orlov SN, Gossard F, Pausova Z, Akimova OA, Tremblay J, Grim CE, Kotchen JM, Kotchen TA, Gaudet D, Cowley AW, Hamet P

MESH terms used to index this publication - Major topics in bold

Adult
Aged
Aged, 80 and over
Animals
Body Mass Index
Dyslipidemias
Erythrocytes
Female
Humans
Hypertension
Lipids
Male
Middle Aged
Rats
Sodium-Potassium-Chloride Symporters
Solute Carrier Family 12, Member 2
Young Adult