Heated lymphocytes express HLA-DR antigens despite their inability to stimulate in MLC. Tissue Antigens 1983 Oct;22(4):246-56
Date
10/01/1983Pubmed ID
6228035DOI
10.1111/j.1399-0039.1983.tb01200.xScopus ID
2-s2.0-0020624495 (requires institutional sign-in at Scopus site) 26 CitationsAbstract
We have utilized serological techniques and mixed lymphocyte culture (MLC) reactions to examine HLA-DR and HLA-D expression by heated (45 degrees C for 1 h) lymphocytes in order to study the functional relationship of these antigens. Heated lymphocytes do not stimulate proliferation of allogeneic lymphocytes in MLC, yet they express HLA-DR antigens. The fraction of peripheral blood lymphocytes (PBL) expressing DR is not altered by heating, nor is the staining intensity altered as detected by fluorescence microscopy. Alloantisera to "B cell alloantigens" recognize HLA-DR determinants on heated cells without any detectable change in either specificity or quantitative cytotoxic effects. Flow cytometry with monoclonal antibody demonstrates only minimal decrease in HLA-DR expression after heating. Thus stimulation in MLC requires more of the stimulating cell than the mere expression of HLA-DR.
Author List
Brewer JA, Hank JA, Wendel T, Schmeling GJ, Blank JL, Morrissey LW, Robins HI, Sondel PMAuthor
Greg J. Schmeling MD Vice Chair, Professor in the Orthopaedic Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
B-LymphocytesCells, Cultured
DNA Replication
Flow Cytometry
Fluorescent Antibody Technique
HLA Antigens
HLA-DR Antigens
Histocompatibility Antigens Class II
Hot Temperature
Humans
Kinetics
Lymphocyte Activation
Lymphocyte Culture Test, Mixed
Lymphocytes
