Cytotoxicity of Clostridium difficile toxin A for human colonic and pancreatic carcinoma cell lines. Cancer Res 1992 Sep 15;52(18):5096-9
Date
09/15/1992Pubmed ID
1516066Scopus ID
2-s2.0-0026793251 (requires institutional sign-in at Scopus site) 12 CitationsAbstract
The use of bacterial exotoxins may constitute novel adjuncts to treatment of gastrointestinal tract malignancies. Clostridium difficile toxin A was evaluated for its cytotoxic effect in vitro on 24 human cell lines and strains including carcinomas of the colon, pancreas, prostate, lung, breast, and lymphoid malignancies, as well as nonmalignant tissues. All nine colon and five pancreas cell lines were extraordinarily sensitive to the cytotoxic effect of Clostridium difficile toxin A at very low concentrations. This effect, which occurred rapidly and was dose dependent, was observed in all cells of seven colon and two pancreas cell lines at concentrations as low as 1-5 ng/ml (10(-12) to 10(-11) M), whereas cells derived from other sites required 60 to greater than 500 ng/ml to achieve an equivalent effect. The data suggest that Clostridium difficile toxin A may have potential therapeutic value in the treatment of some gastrointestinal tract cancers.
Author List
Kushnaryov VM, Redlich PN, Sedmak JJ, Lyerly DM, Wilkins TD, Grossberg SEAuthor
Philip N. Redlich PhD, MD Professor in the Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Bacterial ToxinsCarcinoma
Cell Survival
Colonic Neoplasms
Enterotoxins
Humans
In Vitro Techniques
Pancreatic Neoplasms
Time Factors
Tumor Cells, Cultured
