Growth of AKR T cell leukemia lymphoblasts in medium containing interleukin 2 (IL-2). In Vitro 1983 Oct;19(10):759-66
Date
10/01/1983Pubmed ID
6605296DOI
10.1007/BF02618095Scopus ID
2-s2.0-0020980058 (requires institutional sign-in at Scopus site) 9 CitationsAbstract
Long-term cloned mouse leukemic T cell lines were established in vitro using interleukin-2 (IL-2) conditioned media. The cell lines were tested for retention of both antigenic expression and tumorigenicity, as well as for growth characteristics. Several important findings resulted from these studies. A reliable method was developed for consistent success in the culturing and cloning of leukemic T cell lines. Cultured cells from IL-2-dependent lines were found to retain their original histocompatibility and differentiation antigen characteristics for up to 2 yr. Mortality patterns, comparing long-term cloned leukemic T cell lines with fresh AKR leukemia cells, showed that the cloned cells had greater tumorigenicity. An especially interesting finding was that cell lines established both from different mice and from a single organ of an individual mouse were heterogeneous with respect to antigenic makeup, cell growth kinetics, and tumorigenicity. Finally, because of their dependence on IL-2, the cloned tumor cell lines provided excellent index cells to quantify IL-2 activity.
Author List
Shih CY, Truitt RL, Andreani M, Bortin MMAuthor
Robert L. Truitt PhD Emeritus Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAntigens, Surface
Cell Division
Cell Line
Clone Cells
Culture Media
Histocompatibility Antigens
Interleukin-2
Kinetics
Leukemia, Lymphoid
Mice
Mice, Inbred AKR
T-Lymphocytes
