Physical and chemical interactions between nitric oxide and nitroxides. Biochim Biophys Acta 1994 Dec 15;1201(3):437-41
Date
12/15/1994Pubmed ID
7803474DOI
10.1016/0304-4165(94)90073-6Scopus ID
2-s2.0-0028091445 (requires institutional sign-in at Scopus site) 25 CitationsAbstract
The physical and chemical interaction of nitric oxide (NO) with stable nitroxides have been studied in both aqueous and membrane environments. The ESR spectrum of 3-carbamoyl-2,2,5,5-tetramethyl-3-pyrroline-1- yloxy (CTPO) was observed to broaden upon exposure to NO. This effect can be explained by invoking Heisenberg spin exchange as has been previously reported for molecular oxygen. No loss of total spin was observed negating the possibility of a chemical reaction between NO and CTPO. The extent of signal broadening was proportional to the concentration of NO and can thus be used to monitor NO concentration. We have used this method to observe the partitioning of NO into model membranes. We also report the use of multiquantum ESR to detect directly the effects of NO on the membrane bound spin label 12-doxylstearic acid. This methodology may prove useful for detecting NO in both aqueous and lipid environments and for examining the physical properties of NO within biological membranes.
Author List
Singh RJ, Hogg N, Mchaourab HS, Kalyanaraman BAuthors
Neil Hogg PhD Senior Associate Dean, Professor in the Biophysics department at Medical College of WisconsinBalaraman Kalyanaraman PhD Professor in the Biophysics department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
Cyclic N-OxidesElectron Spin Resonance Spectroscopy
Liposomes
Nitric Oxide
