Nitric oxide regulates adenylyl cyclase activity in rat striatal membranes. J Neurochem 2001 Jun;77(5):1279-84
Date
06/05/2001Pubmed ID
11389178DOI
10.1046/j.1471-4159.2001.00331.xScopus ID
2-s2.0-0035006402 (requires institutional sign-in at Scopus site) 12 CitationsAbstract
The regulation of adenylyl cyclase activity by nitric oxide (NO) was studied in rat (Sprague-Dawley) striatal membranes. Three chemically distinct NO donors attenuated forskolin-stimulated activity but did not alter basal activity. Maximum inhibition resulted in a 50% decrease in forskolin-stimulated activity, consistent with the presence of multiple isoforms of adenylyl cyclase and our previous findings that only the forskolin-stimulated activity of the type-5 and -6 isoform family of enzymes is inhibited by NO. To monitor primarily the type-5 isoform, we examined the ability of NO donors to attenuate D(1)-agonist-stimulated adenylyl cyclase activity. Under those conditions, complete inhibition was observed. The data indicate that NO attenuates neuromodulator-stimulated cAMP signaling in the striatum.
Author List
Hudson TY, Corbett JA, Howlett AC, Klein CAuthor
John A. Corbett PhD Professor in the Biochemistry department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Adenylyl CyclasesAnimals
Colforsin
In Vitro Techniques
Isomerism
Membranes
Neostriatum
Nitric Oxide
Nitric Oxide Donors
Nitrites
Nitroprusside
Penicillamine
Rats
Rats, Sprague-Dawley
S-Nitroso-N-Acetylpenicillamine
