Faculty Collaboration Database

Epiligrin is decreased in papulonodular basal cell carcinoma tumor nest basement membranes and the extracellular matrix of transformed human epithelial cells. Exp Dermatol 1995 Jun;4(3):121-9

Date

06/01/1995

Pubmed ID

7551559

DOI

10.1111/j.1600-0625.1995.tb00235.x

Scopus ID

2-s2.0-0029021464 (requires institutional sign-in at Scopus site)   13 Citations

Abstract

Patients with anti-epiligrin cicatricial pemphigoid have anti-basement membrane autoantibodies that immunoprecipitate a set of disulfide-linked human keratinocyte polypeptides that co-migrate in sodium dodecyl sulfate polyacrylamide gel electrophoresis with the same complex identified by monoclonal anti-epiligrin (P1E1) and monoclonal anti-nicein/kalinin (GB3) antibodies. In an attempt to further compare the reactivity of patient autoantibodies, P1E1 and GB3, these reagents were tested against the tumor nest basement membranes of 7 papulonodular basal cell carcinomas. These studies found that all of these reagents showed markedly decreased or no reactivity against this substrate. Though their concordant lack of reactivity failed to distinguish these antibodies, these studies did identify a significant defect in papulonodular basal cell carcinoma tumor nest basement membranes. Similarly, integrin subunits alpha 6, beta 4, alpha 3, and alpha 2 as well as bullous pemphigoid antigens 1 and 2 (all potential receptors for the extracellular matrix ligands epiligrin and nicein/kalinin) were also reduced in these tumor nest basement membranes. These findings signify an extensive impairment in the lamina lucida of this neoplasm's basement membrane. Related comparative studies of normal human keratinocytes and transformed human epithelial cell lines (specifically, A-431 and HaCat cells) showed that epiligrin production is markedly decreased in the latter. Decreased expression of epiligrin and nicein/kalinin in papulonodular basal cell carcinoma tumor nest basement membranes in vivo and transformed epithelial cells in vitro indicate that this complex is a transformation-sensitive cell adhesion ligand.

Author List

Lazarova Z, Domloge-Hultsch N, Yancey KB

MESH terms used to index this publication - Major topics in bold

Antibodies, Monoclonal
Autoantibodies
Basement Membrane
CD3 Complex
Cell Adhesion Molecules
Cell Line, Transformed
Cells, Cultured
Collagen
Enzyme-Linked Immunosorbent Assay
Epithelium
Extracellular Matrix
Fluorescent Antibody Technique
Humans
Integrins
Keratinocytes
Pemphigoid, Benign Mucous Membrane
Precipitin Tests
Skin Neoplasms