Faculty Collaboration Database

Kinetic and motor functions mediated by distinct regions of the regulatory light chain of smooth muscle myosin. Biochim Biophys Acta 2009 Nov;1794(11):1599-605

Date

07/29/2009

Pubmed ID

19635597

Pubmed Central ID

PMC2757001

DOI

10.1016/j.bbapap.2009.07.009

Scopus ID

2-s2.0-70249148519 (requires institutional sign-in at Scopus site)   3 Citations

Abstract

To understand the importance of selected regions of the regulatory light chain (RLC) for phosphorylation-dependent regulation of smooth muscle myosin (SMM), we expressed three heavy meromyosins (HMMs) containing the following RLC mutants; K12E in a critical region of the phosphorylation domain, GTDP(95-98)/AAAA in the central hinge, and R160C a putative binding residue for phosphorylated S19. Single-turnover actin-activated Mg(2+)-ATPase (V(max) and K(ATPase)) and in vitro actin-sliding velocities were examined for both unphosphorylated (up-) and phosphorylated (p-) states. Turnover rates for the up-state (0.007-0.030 s(-1)) and velocities (no motion) for all constructs were not significantly different from the up-wild type (WT) indicating that they were completely turned off. The apparent binding constants for actin in the presence of ATP (K(ATPase)) were too weak to measure as expected for fully regulated constructs. For p-HMM containing GTDP/AAAA, we found that both ATPase and motility were normal. The data suggest that the native sequence in the central hinge between the two lobes of the RLC is not required for turning the HMM off and on both kinetically and mechanically. For p-HMM containing R160C, all parameters were normal, suggesting that R160C is not involved in coordination of the phosphorylated S19. For p-HMM containing K12E, the V(max) was 64% and the actin-sliding velocity was approximately 50% of WT, suggesting that K12 is an important residue for the ability to sense or to promote the conformational changes required for kinetic and mechanical activation.

Author List

Ni S, Hong F, Brewer PD, Ikebe M, Onishi H, Baker JE, Facemyer KC, Cremo CR

MESH terms used to index this publication - Major topics in bold

Amino Acid Substitution
Animals
Kinetics
Molecular Motor Proteins
Myosin Light Chains
Myosin Subfragments
Phosphorylation
Protein Structure, Tertiary
Smooth Muscle Myosins