Oxidized low density lipoprotein increases U937 cell 5-lipoxygenase activity: induction of 5-lipoxygenase activating protein. Biochem Biophys Res Commun 1994 Jun 15;201(2):1014-20
Date
06/15/1994Pubmed ID
8002971DOI
10.1006/bbrc.1994.1803Scopus ID
2-s2.0-0028308423 (requires institutional sign-in at Scopus site) 18 CitationsAbstract
This study examines the effects of oxidized low density lipoprotein (ox-LDL) on mononuclear cell arachidonic acid (AA) metabolism. U937 and HL60 cells, employed as models for mononuclear cells and promyelocytic cells, respectively, were exposed to ox-LDL (20 micrograms protein/ml) for 24 hours. HPLC analysis of 1-14C-AA metabolites indicates that ox-LDL increases U937 and HL60 cell production of 15-hydroxyeicosatetraenoic acid (15-HETE) and 5-hydroxyeicosatetraenoic acid (5-HETE). Northern analysis indicates that ox-LDL increases U937 cell FLAP transcript levels 10-times control levels but did not appear to alter 5-lipoxygenase (5-LO) mRNA levels. In contrast, ox-LDL increases HL60 cell transcript levels for FLAP and 5-LO 1.5 times and 10 times control levels, respectively. Thus, we propose that ox-LDL plays an important role in the up-regulation of the 5-LO pathway in mononuclear cells. Such activation may explain, in part, the mechanisms by which ox-LDL promotes atherogenesis.
Author List
Fair A, Pritchard KA JrAuthor
Kirkwood A. Pritchard PhD Professor in the Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
5-Lipoxygenase-Activating ProteinsArachidonate 5-Lipoxygenase
Arachidonic Acid
Blotting, Northern
Carrier Proteins
Cell Line
Enzyme Activation
Gene Expression
Humans
Hydroxyeicosatetraenoic Acids
Kinetics
Leukemia
Lipoproteins, LDL
Lymphoma, Large B-Cell, Diffuse
Malondialdehyde
Membrane Proteins
Oxidation-Reduction
Precursor Cell Lymphoblastic Leukemia-Lymphoma
RNA, Neoplasm
Transcription, Genetic
Tumor Cells, Cultured
