Effect of menopause on gene expression profiles of circulating monocytes: a pilot in vivo microarray study. J Genet Genomics 2007 Nov;34(11):974-83
Date
11/27/2007Pubmed ID
18037134DOI
10.1016/S1673-8527(07)60110-6Scopus ID
2-s2.0-36248961034 (requires institutional sign-in at Scopus site) 14 CitationsAbstract
Menopause is one of the key physiological events in the female life and can increase the risk for a number of complex autoimmune, neurodegenerative, metabolic, and cardiovascular disorders. Circulating monocytes can differentiate into various cell types and play an important role in tissue morphogenesis and immune response. We studied gene expression profiles of peripheral blood monocytes in healthy pre- and postmenopausal women using Affymetrix Human U133A GeneChip array that contains probes for approximately 14,500 genes. Comparative analyses between the samples showed that 20 genes were up- and 20 were down-regulated. Of these genes, 28 were classified into six major GO categories relevant to such biological processes as the cell proliferation, immune response, cellular metabolism, and the others. The remaining 12 genes have yet unidentified biological functions. Our results support the hypothesis that functional state of circulating monocytes is indeed affected by menopause, and resulting changes may be determined through the genomewide gene expression profiling. Several differentially expressed genes identified in this study may be candidates for further studies of menopause-associated systemic autoimmune, neurodegenerative, and cardiovascular disorders. Our study is only the first attempt in this direction, but it lays a basis for further research.
Author List
Dvornyk V, Liu Y, Lu Y, Shen H, Lappe JM, Lei S, Recker RR, Deng HMESH terms used to index this publication - Major topics in bold
Blood CirculationFemale
Gene Expression Profiling
Humans
Menopause
Middle Aged
Monocytes
Oligonucleotide Array Sequence Analysis
Pilot Projects
Postmenopause
Premenopause
