Medical College of Wisconsin
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Quantification of human plasma inorganic pyrophosphate. I. Normal values in osteoarthritis and calcium pyrophosphate dihydrate crystal deposition disease. Arthritis Rheum 1979 Aug;22(8):886-91

Date

08/01/1979

Pubmed ID

223577

DOI

10.1002/art.1780220812

Scopus ID

2-s2.0-0018636286 (requires institutional sign-in at Scopus site)   35 Citations

Abstract

The methodologic variables of the UDPG pyrophosphorylase method for analysis of inorganic pyrophosphate (PPi) levels in biologic fluids are described. Use of a tourniquet in collection of blood specimens elevated plasma PPi levels from 35% to 55% above control values and may explain the differences in published normal values. The sodium pyrophosphate decahydrate used to prepare the standard solution lost 8 waters of hydration after dessication, which could result in the calculation of spuriously elevated PPi levels. Normal plasma PPi concentration was 2.18 muM with a range (95% confidence limits) of 0.58-3.78 muM. Comparison of plasma PPi in normal subjects, patients with primary osteoarthritis, and patients with calcium pyrophosphate dihydrate deposition disease revealed no significant intergroup differences.

Author List

Ryan LM, Kozin F, McCarty DJ

Author

Lawrence M. Ryan MD Emeritus Professor in the Medicine department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Blood Specimen Collection
Calcium Pyrophosphate
Chondrocalcinosis
Diphosphates
Humans
Methods
Osteoarthritis
Tourniquets