Cartilage nucleoside triphosphate pyrophosphohydrolase. II. Role in extracellular pyrophosphate generation and nucleotide metabolism. Arthritis Rheum 1985 Apr;28(4):413-8
Date
04/01/1985Pubmed ID
2985090DOI
10.1002/art.1780280409Scopus ID
2-s2.0-0021858466 (requires institutional sign-in at Scopus site) 26 CitationsAbstract
Extracellular generation of inorganic pyrophosphate (PPi) in cartilage organ culture is markedly augmented by ATP.ATP, not an ATP metabolite (ADP, AMP, adenosine) is necessary for this augmentation. Excess PPi production is effectively blocked by known inhibitors of nucleoside triphosphate (NTP) pyrophosphohydrolase (EDTA, EGTA, dithiothreitol). Excess 32P-PPi is generated directly from gamma 32P-ATP by cartilage, as substrate and product have similar specific activities. These findings strongly favor ecto-NTP pyrophosphohydrolase as the source of extracellular PPi generation in the presence of NTP. Additionally, active nucleotide and nucleoside catabolism is demonstrated in these cartilage organ cultures.
Author List
Ryan LM, Wortmann RL, Karas B, McCarty DJ JrAuthor
Lawrence M. Ryan MD Emeritus Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Adenosine TriphosphateAnimals
Cartilage, Articular
Diphosphates
Dogs
Nucleosides
Nucleotides
Organ Culture Techniques
Pyrophosphatases
