Faculty Collaboration Database

The reaction between nitric oxide and alpha-tocopherol: a reappraisal. Biochem Biophys Res Commun 1996 Jul 25;224(3):696-702

Date

07/25/1996

Pubmed ID

8713109

DOI

10.1006/bbrc.1996.1086

Scopus ID

2-s2.0-0030601217 (requires institutional sign-in at Scopus site)   23 Citations

Abstract

Recently Gorbunov et al. reported that nitric oxide (.NO) can directly oxidize alpha-tocopherol to alpha-tocopheroxyl radical (Gorbunov et al., Biochem. Biophys. Res. Commun., 219, 835-841, 1996). We have reinvestigated this reaction and report that a direct reaction between .NO and alpha-tocopherol does not occur. However, the reaction between .NO and oxygen generates an oxidant which oxidizes alpha-tocopherol to alpha-tocopheryl quinone. Exposure of alpha-tocopherol to a low flux of .NO generated from spermine NONOate (100 microM) results in no consumption of alpha-tocopherol under either aerobic or anaerobic conditions. A higher flux of .NO, generated from 1 mM spermine NONOate, oxidizes alpha-tocopherol only under aerobic conditions. Artifactual oxidation of alpha-tocopherol can be observed when using commercial .NO that is contaminated with higher oxides of nitrogen, such as dinitrogen trioxide and dinitrogen tetraoxide.

Author List

Hogg N, Singh RJ, Goss SP, Kalyanaraman B

Authors

Neil Hogg PhD Senior Associate Dean, Professor in the Biophysics department at Medical College of Wisconsin
Balaraman Kalyanaraman PhD Professor in the Biophysics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Free Radicals
Nitric Oxide
Oxidation-Reduction
Oxygen
Vitamin E