Quantitation of Leishmania tropica major by its ability to form distinct colonies on agar-based media. J Parasitol 1983 Dec;69(6):1068-71
Date
12/01/1983Pubmed ID
6674456Scopus ID
2-s2.0-0020959088 (requires institutional sign-in at Scopus site) 14 CitationsAbstract
The growth of the pathogen Leishmania tropica major on agar-based media was studied to further develop techniques that can be used to quantitate the number of viable organisms in infected tissues. Individual, intact amastigotes formed distinct, countable colonies on four different agar-based media. However, the largest colonies (0.35 mm) and highest plating efficiency (greater than 90%) were found on media formulated with a heart-infusion agar base. Use of this media in the quantitation of the organism should increase the sensitivity of the quantitation technique and allow one to collect more interpretable data on host-parasite interactions in experimental cutaneous leishmaniasis.
Author List
Hill JOMESH terms used to index this publication - Major topics in bold
AgarAnimals
Blood
Culture Media
Leishmania
Leishmaniasis
Mice
Mice, Inbred BALB C
Rabbits
