Elimination of CD4+ suppressor T cells from susceptible BALB/c mice releases CD8+ T lymphocytes to mediate protective immunity against Leishmania. J Exp Med 1989 May 01;169(5):1819-27
Date
05/01/1989Pubmed ID
2523955Pubmed Central ID
PMC2189324DOI
10.1084/jem.169.5.1819Scopus ID
2-s2.0-0024564206 (requires institutional sign-in at Scopus site) 103 CitationsAbstract
This study examined the capacity of BALB/c mice that had been depleted of T cell subpopulations to generate a protective immune response to Leishmania major. Thymectomized mice were depleted of either L3T4+ (CD4+) T lymphocytes, Ly2+ (CD8+) T lymphocytes, or both, by treatment with appropriate mAbs. It was found that susceptible mice were rendered resistant to Leishmania by an intravenous infusion of anti-L3T4 mAb. These mice generated an immune response that destroyed the parasite in the primary lesion and in visceral metastatic foci. CD4+ cell-depleted mice also acquired a capacity to mount a sustained delayed-type hypersensitivity (DTH) response to parasite antigens, indicating that DTH, per se, is not a disease-promoting mechanism in the susceptible murine host as has been suggested. Depleting BALB/c mice of CD8+, as well as CD4+ T cells, left them highly susceptible to Leishmania infection, thereby indicating that CD8+ lymphocytes are key protective cells. Our results can be interpreted as showing that the susceptibility of BALB/c mice is due to the generation of CD4+ cells that suppress either the generation or expression of CD8+ T cell-mediated antiLeishmania immunity.
Author List
Hill JO, Awwad M, North RJMESH terms used to index this publication - Major topics in bold
AnimalsAntibodies, Monoclonal
Antigens, Differentiation, T-Lymphocyte
CD8 Antigens
Hypersensitivity, Delayed
Immunization, Passive
Kinetics
Leishmania
Leishmaniasis
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
T-Lymphocytes
T-Lymphocytes, Regulatory
