Faculty Collaboration Database

A reference cross DNA panel for zebrafish (Danio rerio) anchored with simple sequence length polymorphisms. Development 1996 Dec;123:451-60

Date

12/01/1996

Pubmed ID

9007262

DOI

10.1242/dev.123.1.451

Scopus ID

2-s2.0-12644310318 (requires institutional sign-in at Scopus site)   155 Citations

Abstract

The ultimate informativeness of the zebrafish mutations described in this issue will rest in part on the ability to clone these genes. However, the genetic infrastructure required for the positional cloning in zebrafish is still in its infancy. Here we report a reference cross panel of DNA, consisting of 520 F2 progeny (1040 meioses) that has been anchored to a zebrafish genetic linkage map by 102 simple sequence length polymorphisms. This reference cross DNA provides: (1) a panel of DNA from the cross that was used to construct the genetic linkage map, upon which polymorphic gene(s) and genetic markers can be mapped; (2) a fine order mapping tool, with a maximum resolution of 0.1 cM; and (3) a foundation for the development of a physical map (an ordered array of clones each containing a known portion of the genome). This reference cross DNA will serve as a resource enabling investigators to relate genes or genetic markers directly to a single genetic linkage map and avoid the problem of integrating different maps with different genetic markers, as must be currently done when using randomly amplified polymorphic DNA markers, or as has occurred with human genetic linkage maps.

Author List

Knapik EW, Goodman A, Atkinson OS, Roberts CT, Shiozawa M, Sim CU, Weksler-Zangen S, Trolliet MR, Futrell C, Innes BA, Koike G, McLaughlin MG, Pierre L, Simon JS, Vilallonga E, Roy M, Chiang PW, Fishman MC, Driever W, Jacob HJ

MESH terms used to index this publication - Major topics in bold

Alleles
Animals
Crosses, Genetic
DNA Primers
Genetic Markers
Genotype
Polymorphism, Genetic
Reference Standards
Repetitive Sequences, Nucleic Acid
Sequence Analysis, DNA
Zebrafish