Replication of plasmids with the p15A origin in Shewanella putrefaciens MR-1. Lett Appl Microbiol 1997 Mar;24(3):221-5
Date
03/01/1997Pubmed ID
9080705DOI
10.1046/j.1472-765x.1997.00389.xScopus ID
2-s2.0-0030890410 (requires institutional sign-in at Scopus site) 38 CitationsAbstract
The plasmid pACYC184 was introduced into Shewanella putrefaciens MR-1 by electroporation. In 100% of the transformants examined, the plasmid was maintained as a free replicon outside the chromosome. This was the case whether or not the plasmid contained a 224-bp DNA insert derived from an open-reading frame of MR-1 genomic DNA. Therefore, in contrast to a report in the literature, plasmids containing the p15A origin of replication can replicate freely in S. putrefaciens MR-1, and do not make convenient vectors for gene replacement in this bacterium. However, we found that plasmids with the pMB1 origin of replication (e.g. pBR322) cannot replicate in MR-1 and could therefore have potential as vectors for gene replacement.
Author List
Myers CR, Myers JMMESH terms used to index this publication - Major topics in bold
Blotting, SouthernCloning, Molecular
DNA Transposable Elements
Electrophoresis, Agar Gel
Escherichia coli
Gram-Negative Facultatively Anaerobic Rods
Open Reading Frames
Plasmids
Replication Origin
Transformation, Genetic
