Faculty Collaboration Database

Antibodies to a synthetic peptide that react with flavin-containing monooxygenase (HLFMO3) in human hepatic microsomes. J Pharmacol Toxicol Methods 1997 Mar;37(2):61-6

Date

03/01/1997

Pubmed ID

9174980

DOI

10.1016/s1056-8719(97)00012-9

Scopus ID

2-s2.0-0030911445 (requires institutional sign-in at Scopus site)   2 Citations

Abstract

Flavin-containing monooxygenases (FMOs) catalyze the oxidation of a diverse array of xenobiotic compounds. The purpose of this investigation was to develop a specific immunological probe to human hepatic flavin-containing monooxygenase (HLFMO3). An oligopetide corresponding to amino acid residues 257-270 of HLFMO3 was coupled to keyhole limpet hemocyanin (KLH) through the sulfhydryl group of a cysteine residue added to the amino-terminus of the peptide. This peptide-KLH conjugate was used to generate a polyclonal antibody. The resulting immunoglobulin showed specific Western blot reactivity with HLFMO3 protein in human hepatic microsomes, the same protein that is recognized by a polyclonal antibody directed against macaque liver FMO. These findings demonstrate that an antibody directed against a synthetic peptide derived from HLFMO3 can be easily produced in large quantities and used in studies for the immunodetection and immunoquantification of HLFMO3. This is also the first antipeptide antibody directed against an FMO of any species.

Author List

Myers CR, Porgilsson B, Myers JM

MESH terms used to index this publication - Major topics in bold

Animals
Antibodies
Blotting, Western
Female
Humans
Microsomes, Liver
Oxygenases
Peptide Library
Peptides
Rabbits
Sequence Alignment