Faculty Collaboration Database

Paraformaldehyde effect on ruthenium red and lysine preservation and staining of the staphylococcal glycocalyx. Microsc Res Tech 1997 Mar 01;36(5):422-7

Date

03/01/1997

Pubmed ID

9140944

DOI

10.1002/(SICI)1097-0029(19970301)36:5<422::AID-JEMT12>3.0.CO;2-U

Scopus ID

2-s2.0-0030945088 (requires institutional sign-in at Scopus site)   23 Citations

Abstract

The utility of lysine in glutaraldehyde-ruthenium red fixatives for the preservation and/or staining of the fibrous staphylococci glycocalyx was improved by inclusion of paraformaldehyde. Short, 20 min prefixation times for paraformaldehyde-glutaraldehyde fixatives containing lysine, with or without ruthenium red, were compared to an extended overnight fixation. Samples were often lost in fixatives that did not contain paraformaldehyde at extended fixation times hampering the effective use of these fixatives for clinical or environmental applications. Inclusion of paraformaldehyde in the fixation with lysine permitted longer fixation times as well as stabilized the staphylococcal glycocalyx. Thus, the technical usefulness of fixatives employing lysine was significantly improved.

Author List

Fassel TA, Mozdziak PE, Sanger JR, Edmiston CE

Author

James R. Sanger MD Professor in the Plastic Surgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Fixatives
Formaldehyde
Glycocalyx
Lysine
Polymers
Ruthenium Red
Staining and Labeling
Staphylococcus