Faculty Collaboration Database

A microsatellite genetic linkage map for zebrafish (Danio rerio). Nat Genet 1998 Apr;18(4):338-43

Date

04/16/1998

Pubmed ID

9537415

DOI

10.1038/ng0498-338

Scopus ID

2-s2.0-0031897404 (requires institutional sign-in at Scopus site)   313 Citations

Abstract

We have constructed a zebrafish genetic linkage map consisting of 705 simple sequence-length polymorphism markers (SSLPs). The map covers 2350 centimorgans (cM) of the zebrafish genome with an average resolution of 3.3 cM. It is a complete map in genetic mapping terms (there is one linkage group for each of the 25 chromosomes), and it has been confirmed by somatic-cell hybrids and centromere-mapping using half-tetrad analysis. The markers are highly polymorphic in the zebrafish strains used for genetic crosses and provide a means to compare genetic segregation of developmental mutations between laboratories. These markers will provide an initial infrastructure for the positional cloning of the nearly 600 zebrafish genes identified as crucial to vertebrate development,and will become the anchor for the physical map of the zebrafish genome.

Author List

Knapik EW, Goodman A, Ekker M, Chevrette M, Delgado J, Neuhauss S, Shimoda N, Driever W, Fishman MC, Jacob HJ

MESH terms used to index this publication - Major topics in bold

Animals
Chromosome Mapping
Genes
Genetic Linkage
Genetic Markers
Genome
Microsatellite Repeats
Sequence Analysis, DNA
Zebrafish