Cell surface exposure of the outer membrane cytochromes of Shewanella oneidensis MR-1. Lett Appl Microbiol 2003;37(3):254-8
Date
08/09/2003Pubmed ID
12904229DOI
10.1046/j.1472-765x.2003.01389.xScopus ID
2-s2.0-0042378909 (requires institutional sign-in at Scopus site) 107 CitationsAbstract
AIM: To determine if the outer membrane (OM) cytochromes of the metal-reducing bacterium Shewanella oneidensis MR-1 are exposed on the cell surface.
METHODS AND RESULTS: MR-1 cells were incubated with proteinase K or buffer and the resulting degradation of the OM cytochromes was examined by Western blotting. The periplasmic fumarate reductase (control) was not degraded. The OM cytochromes OmcA and OmcB were significantly degraded by proteinase K (71 and 31%, respectively). Immunofluorescence confirmed a prominent cell surface exposure of OmcA and a partial exposure of OmcB and the noncytochrome OM protein MtrB.
CONCLUSIONS: The cytochromes OmcA and OmcB are exposed on the outer face of the OM.
SIGNIFICANCE AND IMPACT OF THE STUDY: The cell surface exposure of these cytochromes could allow them to directly contact extracellular insoluble electron acceptors (e.g. manganese oxides) and is consistent with their in vivo role.
Author List
Myers CR, Myers JMMESH terms used to index this publication - Major topics in bold
Bacterial Outer Membrane ProteinsBacterial Proteins
Cell Membrane
Cytochrome c Group
Endopeptidase K
Fluorescent Antibody Technique
Shewanella
