Cellular localization of androgen and estrogen receptors in mouse-derived motoneuron hybrid cells and mouse facial motoneurons. Dev Neurobiol 2007 Sep 01;67(10):1362-70
Date
07/20/2007Pubmed ID
17638386DOI
10.1002/dneu.20505Scopus ID
2-s2.0-34547755791 (requires institutional sign-in at Scopus site) 12 CitationsAbstract
The ability of gonadal steroid hormones to augment axonal regeneration after peripheral nerve injury has been well established in rat and hamster motoneuron systems, and provides a foundation for the use of these agents as neurotherapeutics. With the advent of mouse genetics and the availability of transgenic and knockout mice, the use of mice in studies of neuroprotection is growing. It has recently been demonstrated that both androgens and estrogens rescue motoneurons (MN) from injury in mouse-derived motoneuron hybrid cells in vitro and mouse facial motoneurons (FMN) in vivo (Tetzlaff et al. [2006] J Mol Neurosci 28:53-64). To elucidate the molecular mechanisms of these effects, the present study examined the cellular localization of androgen and estrogen receptors in mouse MN in vitro and in vivo. Immunoblotting and immunocytochemistry studies established the presence of androgen receptor (AR) and estrogen receptor alpha/beta in immortalized mouse motoneuron hybrid cells and AR and estrogen receptor alpha in mouse FMN.
Author List
Tetzlaff J, Tanzer L, Jones KJMESH terms used to index this publication - Major topics in bold
AnimalsCell Line, Tumor
Cell Survival
Cytoprotection
Estrogen Receptor alpha
Estrogen Receptor beta
Facial Nerve
Gonadal Steroid Hormones
Hybridomas
Mice
Motor Neurons
Nerve Regeneration
Receptors, Androgen
Receptors, Estrogen
Spinal Cord
