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Transforming growth factor beta-1 stimulates articular chondrocyte elaboration of matrix vesicles capable of greater calcium pyrophosphate precipitation. Osteoarthritis Cartilage 2001 Apr;9(3):189-94

Date

04/13/2001

Pubmed ID

11300741

DOI

10.1053/joca.2000.0375

Scopus ID

2-s2.0-0035032346 (requires institutional sign-in at Scopus site)   22 Citations

Abstract

Objective To determine the role of transforming growth factor beta1 (TGFbeta) in early calcium pyrophosphate formation by measuring its effects on articular chondrocyte matrix vesicle (MV) formation, specific activity of the inorganic pyrophosphate(PPi)-generating enzyme nucleoside triphosphate pyrophospho-hydrolase (NTPPPH) and biomineralization capacity. Methods MV elaborated from mature porcine chondrocyte monolayers+/-TGFbeta were compared for protein content, NTPPPH activity, and ATP-dependent biomineralization. Precipitation of calcium pyrophosphate mineral phases by MV was determined by a radiometric assay and by Fourier transform infrared spectroscopy (FTIR). Results MV from monolayers exposed to TGFbeta were enriched in NTPPPH activity compared to MV from control monolayers (P< 0.01) and precipitated more calcium/mg MV protein than controls (P</= 0.01). FTIR spectra of mineral generated by monolayer-elaborated MV were consistent with poorly crystalline CPPD. Conclusions TGFbeta is capable of increasing the capacity of articular chondrocyte-derived MV to generate PPi via NTPPPH and precipitate calcium in the form of CPPD mineral. These data support the concept that this growth factor plays a key role in cartilage matrix CPPD deposition.

Author List

Derfus BA, Camacho NP, Olmez U, Kushnaryov VM, Westfall PR, Ryan LM, Rosenthal AK

Authors

Ann K. Rosenthal MD Associate Dean, Professor in the Medicine department at Medical College of Wisconsin
Lawrence M. Ryan MD Emeritus Professor in the Medicine department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Bone Matrix
Calcification, Physiologic
Calcium Pyrophosphate
Cartilage, Articular
Chondrocytes
Pyrophosphatases
Swine
Transforming Growth Factor beta