Downregulation of L3T4+ cytotoxic T lymphocytes by interleukin-2. Science 1987 Oct 16;238(4825):344-7
Date
10/16/1987Pubmed ID
2443976DOI
10.1126/science.2443976Scopus ID
2-s2.0-0023231426 (requires institutional sign-in at Scopus site) 11 CitationsAbstract
Proliferation of activated cytotoxic T lymphocytes (CTLs) that recognize foreign histocompatibility antigens is induced by interleukin-2, a potent immunoregulatory molecule originally described as T cell growth factor. Interleukin-2 (IL-2) is widely used to isolate and induce clonal expansion of CTLs for functional studies in vitro and in vivo. However, in studies with CTLs specific for class I and class II histocompatibility antigens, IL-2 rapidly downregulated the lytic activity of some class II-specific CTLs in a time- and dose-dependent manner. Lytic activity of L3T4+ CTLs specific for the murine class II antigen I-Ek was repeatedly up- and downregulated in vitro by alternate exposure to specific (alloantigen) and nonspecific (recombinant IL-2) signals, respectively. These results demonstrate that some CTLs modulate their functional property (cytolysis) while undergoing IL-2-driven cell proliferation without loss of antigen specificity or ability to revert to a lytic phenotype.
Author List
Shih CC, Truitt RLAuthor
Robert L. Truitt PhD Emeritus Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAntigens, Differentiation, T-Lymphocyte
Cell Line
Clone Cells
Cytotoxicity, Immunologic
Epitopes
H-2 Antigens
Interleukin-2
Isoantigens
Lymphocyte Activation
Mice
Phenotype
T-Lymphocytes, Cytotoxic
