Transcriptome analysis and kidney research: toward systems biology. Kidney Int 2005 Jun;67(6):2114-22
Date
05/11/2005Pubmed ID
15882254DOI
10.1111/j.1523-1755.2005.00315.xScopus ID
2-s2.0-23044515192 (requires institutional sign-in at Scopus site) 23 CitationsAbstract
An enormous amount of data has been generated in kidney research using transcriptome analysis techniques. In this review article, we first describe briefly the principles and major characteristics of several of these techniques. We then summarize the progress in kidney research that has been made by using transcriptome analysis, emphasizing the experience gained and the lessons learned. Several technical issues regarding DNA microarray are highlighted because of the rapidly increased use of this technology. It appears clear from this brief survey that transcriptome analysis is an effective and important tool for question-driven exploratory science. To further enhance the power of this and other high throughput, as well as conventional approaches, in future studies of the kidney, we propose a multidimensional systems biology paradigm that integrates investigation at multiple levels of biologic regulation toward the goal of achieving a global understanding of physiology and pathophysiology.
Author List
Liang M, Cowley AW Jr, Hessner MJ, Lazar J, Basile DP, Pietrusz JLAuthors
Allen W. Cowley PhD Professor in the Physiology department at Medical College of WisconsinMartin J. Hessner PhD Professor in the Pediatrics department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AnimalsGene Expression Profiling
Humans
Kidney
Oligonucleotide Array Sequence Analysis
Transcription, Genetic
