Post-embedding direct immunogold detection of a protein antigen in insect tissue. J Histochem Cytochem 1986 Feb;34(2):221-6
Date
02/01/1986Pubmed ID
3511140DOI
10.1177/34.2.3511140Scopus ID
2-s2.0-0022626057 (requires institutional sign-in at Scopus site) 5 CitationsAbstract
An immunocytochemical study was performed to localize the site of hemoglobin synthesis in larvae and embryos of the insect Chironomus thummi. Heterologous antisera specific for C. thummi hemoglobins were prepared using a highly purified hemoglobin extract. Tissue samples were prepared by glutaraldehyde fixation of whole dissected larvae or whole embryos without osmium tetroxide postfixation. Epoxy resin-embedded thin sections were labeled with a direct immunogold conjugate. Immune label was localized in rough endoplasmic reticulum of fat body cells of larvae. Immune label was also present in embryos. The technique, which did not require chemical etching of the sections, proved very useful for demonstration of this intracellular protein antigen.
Author List
Myers CR, Bergtrom G, Crook SM, Boyer DR, Collins MLMESH terms used to index this publication - Major topics in bold
Adipose TissueAnimals
Antigens
Chironomidae
Diptera
Gold
Hemoglobins
Histocytochemistry
Immunoenzyme Techniques
Larva
Mononuclear Phagocyte System
