CURRICULUM VITAE
Stephen A. Duncan PhD
Stephen A. Duncan PhD
Professor
Department of Cell Biology, Neurobiology and Anatomy
Department of Cell Biology, Neurobiology and Anatomy
OFFICE ADDRESS: |
Medical Education Building |
8701 Watertown Plank Rd |
Milwaukee, WI 53226 |
Fax: 414-456-6517 |
Email: duncans@mcw.edu |
PLACE OF BIRTH: Glasgow, Scotland, U.K. |
CITIZENSHIP: U.K. and U.S. |
EDUCATION: |
1985 - 1988 B.Sc (hons), University of Glasgow |
1988 - 1992 D.Phil., University of Oxford, Wolfson College |
POSTGRADUATE TRAINING AND FELLOWSHIP APPOINTMENTS: |
1992 - 1995 Postdoctoral fellow, mentor James Darnell, Rockefeller University, New York, NY |
1995 - 1996 Research Associate, Rockefeller University, |
MILITARY SERVICE: |
N/A |
FACULTY APPOINTMENTS: |
1996 - 1997 Assistant Professor, Rockefeller University, New York, NY |
1997 - 2002 Assistant Professor, Cell Biology Neurobiology and Anatomy, Medical College Wisconsin, Milwaukee, WI |
2002 - 2006 Associate Professor, Cell Biology Neurobiology and Anatomy, Medical College Wisconsin, Milwaukee, WI |
2006 - Present Professor, Cell Biology Neurobiology and Anatomy, Medical College Wisconsin, Milwaukee, WI |
2007 - Present Marcus Professor of Human and Molecular Genetics., Cell Biology Neurobiology and Anatomy, Medical College Wisconsin, Milwaukee, WI |
ADMINISTRATIVE APPOINTMENTS: |
2007 - Present Director Program in Regenerative Medicine/Stem Cell Biology, Cell Biology Neurobiology and Anatomy, Medical College Wisconsin, Milwaukee, WI |
2009 - Present Vice Chairman, Cell Biology Neurobiology and Anatomy, Medical College Wisconsin, Milwaukee, WI |
AWARDS AND HONORS: |
1988 - 1991 MRC AIDS Directed Program Research Studentship |
1995 - 1998 Naomi Judd American Liver Scholar |
1995 - 1998 Alexander and Alexandrine Sinsheimer Scholar Award |
2009 Wisconsin BizTimes 'Healthcare Hero Grand Achievement" Award |
MEMBERSHIPS IN HONORARY AND PROFESSIONAL SOCIETIES: |
2000 - Present Society for Developmental Biology |
2001 - Present FASEB |
2001 - Present American Society for Microbiologists |
2003 - Present American Association for Cell Biology |
2004 - Present American Association for Advancement in Science |
2010 - Present International Society for Stem Cell Research |
EDITORSHIPS/EDITORIAL BOARDS/JOURNAL REVIEWS: |
Editorial Board |
2005 - Present BMC Developmental Biology |
2006 - Present Developmental Dynamics |
Journal Review |
Molecular and Cellular Biology |
Journal of Cell Biology |
Developmental Dynamics |
Hepatology |
Gastroenterology |
Cell Stem Cell |
Journal of Hepatology |
Nature |
Developmental Biology |
Nature Reviews |
Proceedings of the National Academies of Science, USA |
BMC Developmental Biology |
Development |
Nucleic Acid Research |
Mechanisms of Development |
Nature Medicine |
EMBO Reports |
Nature Genetics |
Science |
Genes and Development |
European Molecular Biology Organization (EMBO) Journal |
LOCAL/REGIONAL APPOINTED LEADERSHIP AND COMMITTEE POSITIONS: |
2002 - 2011 Board of Directors, Wisconsin Association for Biomedical Research and Development |
NATIONAL ELECTED/APPOINTED LEADERSHIP AND COMMITTEE POSITIONS: |
2000 Member, Review Panel, site visit review of Program Project Grant (P01), NIH |
2000 Reviewer, Ad Hoc Reviewer, Institut Pastuer, Unite de Genetique e la Differentiation, Institut Pastuer |
2000 Reviewer, Grant Reviews, Human Frontier Science Program |
2001 Reviewer, Actions de Recherche Concertees, Universite de Louvain, Belgium |
2001 Reviewer, Member, NIH NIDDK Special Study Section, RFA " Development of gut, liver and exocrine pancreas", NIDDK |
2003 Reviewer, Grant Reviews, Welcome Trust |
2003 Reviewer, Grant Reviews, National Science Foundation |
2003 Reviewer, NIH Metabolism Study Section, NIH |
2004 Reviewer, Reviewer trans-NIH “Action Plan for Liver Disease Research”., NIH |
2004 Reviewer, Dev2 Study Section, NIH |
2004 Organizer, FASEB Summer Research Conference, Mechanisms of Liver Growth, Development and Disease, July 2006, Snowmass, Colorado, FASEB |
2005 - 2009 Member, Dev2 Study Section, National Institutes of Health |
2005 Reviewer, Dev2 Study Section, NIH |
2005 Reviewer, External Review of Genetic Expression and Disease Unit., Institut Pastuer |
2006 Reviewer, Ex Ante International Expert Evaluator, Belgian Science Policy “Interuniversity Attraction Poles” programme (IAP, 2007-2011) |
2006 Reviewer, Grant Review, Istituto Pasteur-Fondazione Cenci Bolognettiis |
2007 Co-organizer, Experimental Biology Symposium, “"Early Decisions by Embryonic Stem Cells: Diversification into, and Interaction Between, Endodermal & Mesodermal Lineages", 5-9th April, 2008 |
2007 Reviewer, Actions de Recherche Concertées Application 2007-2012 Pathogenic mechanisms of developmental diseases, UCL, Belgium., UCL |
2008 Reviewer, Grant Reviews, Singapore Biomedical Research Council |
2009 - Present Contributer, Liver Disease Work Group of the International Society for Stem Cell Research Clinical Translation committee., ISSCR |
2009 - 2011 Member - Scientific Advisory Board, Mini-Liver Center Advisory Board., Mount Sinai School of Medicine |
2010 Chairman, Program Project Grant Special Emphasis Panel, NICHD |
2010 - Present Member - Scientific Advisory Board, Center for Digestive and Liver Diseases External Advisory Board., University of Pennsylvania |
2010 Reviewer, Program Project Grant Special Emphasis Panel, NIGMS |
2011 - Present Member - Scientific Advisory Board, Malaria Culture Systems Consortium , Bill & Melinda Gates Foundation |
2012 Reviewer, Special Emphasis Panel, NIH/CSR |
2013 - Present Member - Scientific Advisory Board, Program Project Grant External Scientific Advisory Board, University of Pittsburgh |
RESEARCH GRANTS/AWARDS/CONTRACTS/PROJECTS: | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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INVITED LECTURES/WORKSHOPS/PRESENTATIONS: |
Local |
1. The role of HNF4a in regulation of gene expression and glucose homeostasis, MCW/Max McGee Center 2nd Annual Diabetes Day , MCW, 1/26/2008 - Present |
2. Recent Advances in Embryonic Stem Cell Biology, MCW/Marquette Alumni Weekend Clinic Day , MCW, 5/2008 - Present |
3. Use of induced pluripotent stem cells in treatment and study of disease, MCW Technology Innovation Council Board Meeting, MCW, 1/2009 - Present |
4. Use of Stem Cells in the Treatment of Disease, Rotary Club of Milwaukee, Milwaukee, 7/2009 - Present |
5. Use of Pluripotent Stem Cells in the Study of Disease and Development” , North Shore Rotary Club , Milwaukee, 4/2011 - Present |
6. Use of Pluripotent Stem Cells in the Study of Disease and Development” , Lake Country Rotary Club Eastern WI District Conference , Milwaukee, 5/2011 - Present |
7. Using human induced pluripotent stem cells to study liver development and disease, MCW Endocrine Research Grand Rounds, MCW, 5/10/2012 - Present |
8. Stem Cells & Regenerative Medicine: From here to there...and back again., Milwaukee School of Engineering, My Genes, My Health Science Café, Milwaukee, 11/13/2012 - Present |
9. Advancing Medicine through Stem Cell Research, MCW Council Meeting, University Club of Milwaukee, 1/16/2013 - Present |
10. Using human iPS cells to study liver disease and development., Milwaukee School of Engineering, Biomolecular Engineering program , Milwaukee, 1/21/2013 - Present |
Regional |
1. Molecular Control of Hepatic Development in the Mouse, Seminar, Department of Cell BIology and Anatomy, University of Wisconsin Madison, 1998 |
2. Transcriptional Regulation of Hepatic Development, Endocrinology Grand Rounds , University of Wisconsin Medical College, 3/2002 |
3. Control of Liver Development by Gata Factors and Hnf4, Genetics in model organisms colloquium, Department of Genetics and Biotechnology University of Wisconsin Madison, 1/2004 |
4. Gata Factors in Gut and Liver Development, Seminar, Department of Cell bBiology and Anatomy, University of Wisconsin Madison, 11/2004 |
5. Regulation of Liver and Heart Development By Gata4 and Gata6, Seminar, Department Pathology & Laboratory Medicine, University of Wisconsin Madison, 2/2008 |
6. ES cells as a model of liver development and disease, Madison Campus Stem Cell Laboratory Meeting, University of Wisconsin Madison, 5/2008 |
National |
1. Control of liver and heart development by GATA4 and GATA6, Seminar, Gladstone Institute/UCSF , 2/2007 |
2. Control of liver and heart development by GATA4 and GATA6, Seminar, University of Southern California , 3/2007 |
3. Control of liver and heart development by GATA4 and GATA6, Seminar, Duke University, 3/2007 |
4. Control of liver and heart development by GATA4 and GATA6, Robert Costa Memorial Symposium , University of Illinois Chicago, 3/2007 |
5. Control of liver development and function by HNF4, Seminar, Cleveland Clinic , 5/2007 |
6. ES cells as a model of liver development and disease, Seminar, Einstein Yeshiva University New York, 2/2008 |
7. Control of Cardiac Development by GATA4 and GATA6, Penn Cardiovascular Institute Symposium , University of Pennsylvania, Philidelphia, 5/2008 |
8. Embryonic stem cells and induced pluripotent stem cells as a model of human liver development and disease, FASEB Summer Research Conference: "Liver Growth, Development and Disease". , Snowmass Village, Colorado, 8/2008 |
9. Control of cardiac development by GATA factors, Cardiac Development and Regeneration Abcam Research Conference, Boston, 10/2008 |
10. Analysis of Liver Development and Disease using Human ES cells and iPS cells, Yale University Liver Center Retreat , 11/2008 |
11. Human ES cells and iPS cells as a model of human liver disease and development, Seminar, University of Nebraska, 3/2009 |
12. Efficient Generation of Hepatocytes from Human iPS cells, Seminar, University of California Riverside , 3/2009 |
13. Efficient Generation of Hepatocytes from Human iPS cells, Columbia University, New York City, 10/2009 |
14. Use of human pluripotent stem cells to study liver disease and development, Seminar, Children’s Hospital Medical Center Cincinnatti, 3/2010 |
15. Human iPS cells for study of Liver Disease and Development, Robert Costa Memorial Lecture , University of Illinois Chicago, 4/2010 |
16. Use of human pluripotent stem cells in the study of hepatocyte differentiation and disease, 3rd Annual Southeast Regional Stem Cell Consortium , Vanderbilt University, Nashville, 7/2010 |
17. Study of human liver development and disease using pluripotent stem cells, FASEB Summer Research Conference: "Liver Growth, Injury and Metabolism: Basic and Applied Biology"., Snowmass Village, Colorado, 8/2010 |
18. Human pluripotent stem cells in the study of liver disease and development, Seminar, St Jude’s Memphis, 9/2010 |
19. Pluripotent human stem cells in the study of hepatic disease and development, Visiting Lecturer in the NIH-funded Digestive Disease Center (DDC) at Stanford University , Stanford University, California, 11/2010 |
20. Modeling Liver Disease Using Patient Specific iPS cells, Seminar, University of Miami, 2/2011 |
21. iPS cells as a model to study liver diseases, Invited Speaker Henry M and Lillian Stratton Basic Research Conference/ American Association of the Study of Liver Diseases (AASLD) Single Topic Conference Title: Stem cells in Liver Diseases and Cancer: Discovery and Promise, Atlanta, Georgia, 3/2011 - 2/2011 |
22. Using Human Pluripotent Stem Cells to Study Hepatic Disease and Development, Student’s Choice Seminar, University of Washington , University of Washington, Seattle, WA, 4/2011 |
23. Using Human Pluripotent Stem Cells to Study Hepatic Disease and Development, University of Pennsylvania Digestive Disease Center Symposium , University of Pennsylvania, Philidelphia, 4/2011 |
24. Generation of Hepatocyte-like Cells from Induced Pluripotent Stem Cells, Digestive Disease Week, Research Symposium ‘Endodermal Organ Development and Stem Cell Nuclear Programming”, Chicago, Illinois, 5/2011 |
25. Using human iPS cells to study hepatocyte differentiation and liver disease., Seminar, University of Georgia, Athens , 10/2011 |
26. The use of pluripotent stem cells in the study of liver development and disease, Seminar, University of Kansas Medical Center, 12/2011 |
27. University of Massachusetts Amherst, Modeling liver development and disease with human iPSC derived hepatocytes, 1/2012 |
28. Pluripotent stem cells in the study of liver disease and development, Seminar, University of Pittsburgh, Pittsburgh, 2/2012 |
29. Using Human Pluripotent Stem Cells to Understand Liver Development and Disease, Seminar, University of Minnesota, Minnesota, 2/2012 |
30. Center for the Study of Hepatitis C - Seminar, Rockefeller University, New York City, NY, 3/2012 |
31. “iPS CELLS: Are We There Yet!” , FASEB Experimental Biology 2012 , San Diego, 4/21/2012 - Present |
32. The use of human pluripotent stem cells to study liver disease and development., Visiting Professor at the UCSF Liver Center, San Francisco, 5/2/2012 - Present |
33. Two presentations “Endoderm Regionalization” and “iPSCs and their Application”, Cold Spring Harbor Laboratory and the Mouse Development, Stem Cells & Cancer course, June 6 – 26, 2012. , CHSL, New York, 6/11/2012 - Present |
34. Using Human iPSCs to study inborn errors of hepatic metabolism, 2012 FASEB Summer Research Conference on “Liver Biology: Fundamental Mechanisms & Translational Applications”., Snowmass, Colorado, 7/29/2012 - Present |
35. Human pluripotent stem cells in the study of liver disease and development, Global Health Seminar Series, University of South Florida College of Medicine , Tampa, FL, 12/4/2012 - Present |
36. Stephen Duncan, Pluripotent Stem Cell Derived Hepatocytes Recapitulate the Pathophysiology of Familial Hypercholesterolemia, 3rd Stem Cell Meeting for Drug Developers, Boston, MA, USA, 3/19/2013 - 3/21/2013 |
International |
1. “8th Biennial International Conference on Liver Development, Gene regulation and Disease” , Orvieto, Italy, 6/2/1999 - 1999 |
2. Molecular Regulation of Hepatic Development in Mammals, Edinburgh University, UK, 1999 |
3. Transcription Factors That Regulate Liver Development, Louis Pastuer Institute, France, 1999 |
4. Role of HNF-4 in Cell Differentiation and Development, Cochin Institute, Paris, France, 1999 |
5. Italian Federation of Life Science Congress - Liver development and Disease, Riva del Garda, Italy, 2000 |
6. HNF4 controls development of a hepatic epithelium and liver morphogenesis, EMBO Workshop Liver Development, Gene Regulation and Disease, Herkalion, Greece, 6/2003 |
7. Transcriptional control of liver development, Seminar, Institute Pasteur, Paris, France, 2003 |
8. International Symposia on Developmental Biology and Tissue Engineering, Tokyo Japan., 2003 |
9. What makes a cell into a hepatocyte and a hepatocyte into a liver, Invited State of the Art Lecture, The European Association for the Study of the Liver, Berlin, Germany 2004, 4/2004 |
10. Control of Liver Development by GATA factors and HNF4, Seminar, British Columbia, Canada, 5/2004 |
11. 'Transcriptional control of mammalian hepatic development' , Seminar, Umeå Centre for Molecular Medicine Umeå University, Sweden, 12/2004 |
12. Use of Embryonic Stem Cells to Study Development of the Mammalian Liver, Bioscience 2005 , Scottish Exhibition and Conference Center, Glasgow, UK, 7/2005 |
13. Falk-Workshop, Morphogenesis and Cancerogenisis of the Liver, Gottingen, Germany, 1/2007 |
14. International Symposium on Developmental Biology and Tissue Engineering, Tokyo, Japan, 4/2007 |
15. EMBO Workshop The role and control of GATA factors in tissue development and disease, Capri, Italy, 4/2007 |
16. State-of-the-Art Lecture, Molecular Mechanisms Underlying Mammalian Development, 10th International Workshop on Developmental Biology, Pecs, Hungary, 8/2007 |
17. Using Human iPS cells to Study Liver Disease and Development., UNIA Workshop: Liver and Pancreas: From Development to Disease, Baeza, Spain, 10/2011 |
18. Using Human iPS cells to Study Liver Disease and Development., Liver Down Under meeting, Liver Development, Disease and Regeneration, Perth, Australia, 11/2011 |
19. Use of Human Embryonic Stem Cells to Study Liver Disease and Hepatocyte Differentiation, Progress to Therapy 2012, Glasgow, UK, 3/2012 |
COMMITTEE SERVICE: |
Medical College |
1998 - Present Organizer, MCW Developmental Biology and Stem Cell Forum |
1998 - 1999 Member - Scientific Advisory Board, Interdisciplinary Graduate Program (IDP) Revision Committee |
1998 - 1999 Member - Scientific Advisory Board, IDP Curriculum Revision Committee |
1999 - 2001 Member, Graduate Student Awards Committee |
1999 - 2003 Member, IDP Executive Evaluation Committee |
1999 - 2010 Member, Graduate Studies Council |
1999 - 2002 Member, IDP Web-site committee |
2000 Member, Animal House Expansion Committee |
2000 - 2005 Member, Barrier Facility Committee |
2000 - 2005 Member, Transgenic Facility Oversight Committee |
2002 - 2005 Chairman, Chairman, Graduate Curriculum and Programs Committee |
2003 - Present Member, Graduate Program in Cell Biology Oversight Committee |
2003 Member, Dept Cell Biology Faculty Search Committee |
2003 - 2008 Chairman, IDP Executive Evaluation Committee |
2004 Member, Student Assessment Committee |
2004 - 2005 Member, Search Committee for Associate Director MCW Human and Molecular Genetics Center |
2004 - 2010 Member, Children's Research Institute Scientific Advisory and Policy Committee |
2004 Chairman, Graduate Studies Councinl Bound Thesis Committee |
2004 Member, Department of Cell Biology Faculty Search Committee |
2004 Chairman, MCW Digestive Disease Center Grant Review Committee |
2005 - 2009 Member, Children's Research Institute Advisory Board Subcommittee for Basic Research |
2005 - 2007 Member, Search Committee for Chairman Department. Pathology MCW |
2005 Chairman, Chairman Department of Cell Biology Faculty Search Committee |
2006 Member, Search Committee for Head of Biomedical Research Center |
2006 - 2009 Member, IDP Graduate Program Oversight Committee |
2007 Member, Ad Hoc Graduate Curriculum and Programs Committee |
2007 Chairman, Regenerative Medicine Faculty Search Committee |
2008 Chairman, Regenerative Medicine Faculty Search Committee |
2009 Member, Biospecimen organizing committee |
2009 - Present Member, MCW Biomedical Resource Center Oversight Committee |
2009 Chairman, Task force to define MCW stance on Stem Cell Research |
2009 - Present Member, MCW Cardiovascular Center Scientific Advisory Board |
2009 - Present Member, MCW Embryonic Stem Cell Research Oversight Committee |
2010 - Present Member, MCW Neuroscience Center Scientific Advisory Board |
2010 - 2011 Chairman, Chairman IDP Curriculum and Oversight Committee |
2010 Member, Search Committee Children’s Research Institute/MCW Chief of Pediatric Hematology Oncology |
2011 Member, Rank and Tenure Committee |
EXTRAMURAL TEACHING (e.g. at Marquette, UWM, etc.): |
Medical Student Education |
1999 - 2009 Medical College Wisconsin, Cell and Tissue Biology Lecture - Pancreas |
1999 - 2010 Medical College Wisconsin, Cell and Tissue Biology Course Digestive System 3 "Liver and Pancreas" 2-day lab |
1999 - 2010 Medical College Wisconsin, Cell and Tissue Biology Course - Digestive System 1 "Salivary Glands etc" 2-day lab |
1999 - 2009 Medical College Wisconsin, Cell and Tissue Biology Lecture - Liver and Gallbladder |
1999 - 2010 Medical College Wisconsin, Cell and Tissue Biology Course Digestive System 2 "Small and Large Intestine" 2-day lab |
2002 - 2009 Medical College Wisconsin, Cell and Tissue Biology Lecture - Lower GI |
2002 - 2009 Medical College Wisconsin, Cell and Tissue Biology Lecture - Upper GI |
2010 - Present Medical College Wisconsin, Cell and Tissue Biology Lecture - Stem Cells |
Graduate Student Education |
1998 Medical College Wisconsin, Advanced Cell Biology - 3 sessions |
1998 Medical College Wisconsin, Advanced Genetics - 5 sessions |
1999 Medical College Wisconsin, Molecular Cell Biology - 1 lecture |
1999 - 2007 Medical College Wisconsin, Molecular Genetics - 3 lectures |
2000 Medical College Wisconsin, Course Director - Analysis Communication Scientific Data |
2001 Medical College Wisconsin, Advanced Cell and Development - 7 sessions |
2004 Medical College Wisconsin, Advanced Genetics - 7 sessions |
2005 Medical College Wisconsin, Advanced Cell and Development - 4 sessions |
2006 - 2009 Medical College Wisconsin, Co-director Molecular Cell Biology |
2006 Medical College Wisconsin, Advanced Genetics - 4 sessions |
2007 Medical College Wisconsin, Advanced Cell Biology - 3 sessions |
2007 Medical College Wisconsin, Course Director Advanced Cell and Development |
2008 - Present Medical College Wisconsin, Developmental and Stem Cell Biology - 4 lectures |
2008 - Present Medical College Wisconsin, Director Developmental and Stem Cell Biology |
2010 - Present Medical College Wisconsin, Methods in Grant Preparation - 1 lecture |
2011 Medical College Wisconsin, Director - Stem Cells in Disease and Development |
2011 - Present Medical College Wisconsin, Stem Cells in Disease and Development - 18 sessions |
2012 - Present Medical College Wisconsin, Models of Disease and Drug Discovery - 1 lecture |
EXTRAMURAL STUDENTS, FACULTY, RESIDENTS, AND CLINICAL/RESEARCH FELLOWS MENTORED: |
Graduate Students |
PhD Students Advised |
Jamil Haque, Medical College Wisconsin, 1999 - 2004 Role of Pes1 Gene in Mouse Cell Proliferation |
Wendy Garrison, Medical College Wisconsin, 2000 - 2005 HNF4a Regulates Development of the GI Tract |
Jennifer Luebke-Weeler, Medical College Wisconsin, 2001 - 2007 Creb3l3 is Regulated by HNF4a in the Mouse Embryonic Liver and is Dispensable for Hepatogenesis |
Kai Sun, Medical College Wisconsin, 2001 - 2007 PES1 is Required for Cell Proliferation; Serum Response Factor is Essential for Hepatocyte Proliferation and Liver Function |
Fallon Noto, Medical College Wisconsin, 2007 - 2012 Investigating The Therapeutic Potential Of Induced Pluripotent Stem Cells In A Mouse Model Of Liver Dysfunction |
Ann DeLaForest, Medical College Wisconsin, 2008 - Present |
Max Cayo, Medical College Wisconsin, 2009 - Present |
Kirk Twaroski, Medical College Wisconsin, 2010 - Present |
Megan Determan, Medical College Wisconsin, 2011 - Present |
Amanda Urick, Medical College Wisconsin, 2011 - Present |
Ran Jing, Medical College Wisconsin, 2012 - Present |
MS Students Advised |
Jackie Tekiela, Medical College Wisconsin, 2006 - 2008 |
Postdoctoral Students |
Roong Zhao, Medical College Wisconsin, 1998 - 2006 Mentor |
Fereshteh Parviz, Medical College Wisconsin, 1998 - 2003 Mentor |
Alexandra Lerch, Medical College Wisconsin, 1999 - 2005 Mentor |
Alistair Watt, Medical College Wisconsin, 2000 - 2003 Mentor |
Dong Zhao, Medical College Wisconsin, 2003 - 2006 Mentor |
Chhinder Shodhi, Medical College Wisconsin, 2003 - 2006 Mentor |
Michele Battle, Medical College Wisconsin, 2003 - 2009 Mentor |
Genevieve Konopka, Medical College Wisconsin, 2004 - 2006 Mentor |
Karim Si–Tayeb, Medical College Wisconsin, 2005 - 2011 Mentor |
Masato Nagaoka, Medical College Wisconsin, 2007 - 2012 Mentor |
Jun Cai, Medical College Wisconsin, 2009 - Present Mentor |
Joseph Fisher, Medical College Wisconsin, 2011 - Present Mentor |
Sunil Mallanna, Medical College Wisconsin, 2012 - Present Mentor |
Clinical/Research Fellows |
Nur Actay, Medical College Wisconsin, 1998 - 2000 Mentor |
PROGRAMMATIC DEVELOPMENTS: |
Educational Programs |
Student |
1998 - Present Director Developmental and Stem Cell Biology Forum |
Research Programs |
2007 - Present Regenerative Medicine Research Program |
BIBLIOGRAPHY |
Refereed Journal Publications/Original Papers |
1. Kerr, S. M., L. H. Johnstone, M. Odell, S. A. Duncan, K. M. Law and G. L. Smith. (1991). Vaccinia DNA ligase complements Saccharomyces cerevisiae cdc 9, localizes in cytoplasmic factories and affects virulence and virus sensitivity to DNA damaging agents. EMBO J. 10: 4343-4350. |
2. Duncan, S. A. and G. L. Smith. (1992). Identification and characterization of an extracellular envelope glycoprotein affecting vaccinia virus egress. J. Virol. 66: 1610-1621. |
3. Duncan, S. A. and G. L. Smith. (1992). Vaccinia gene SalF5R is nonessential for virus replication in vitro and in vivo. J. Gen. Virol. 73, 1235-1242. |
4. Duncan, S. A., K. Manova, W. Chen, P. Hoodless, D. C. Weinstein, R. F. Bachvarova, and J. E. Darnell, Jr. (1994). Expression of transcription factor HNF-4 in the extraembryonic endoderm, gut and nephrogenic tissue of the developing mouse embryo: HNF-4 is a marker for primary endoderm in the implanting blastocyst. Proc. Natl. Acad. Sci. USA. 91, 7598-7602. |
5. Chen, W. S., K. Manova, D. C. Weinstein, S. A. Duncan, A. S. Plump, V. R. Prezioso, R. F. Bachvarova, and J. E. Darnell, Jr. (1994). Disruption of the HNF-4 gene, expressed in visceral endoderm, leads to cell death in embryonic ectoderm and impaired gastrulation of mouse embryos. Genes. Dev. 8, 2466-2477 |
6. Chen, J., S. Chang, S. A. Duncan, G. Fishell, and A. Aderem (1995). Disruption of the MacMARKS gene prevents anterior neuropore closure and results in anencephaly. Proc. Natl. Acad. Sci. USA 93, 6275-6279 |
7. Duncan, S. A., Z. Zhong, Z. Wen and J. E. Darnell, Jr. (1997) STAT signaling is active during early mammalian development. Dev. Dyn. 208:190-198 |
8. Duncan, S. A., A. Nagy, and W. Chan (1997) Murine gastrulation requires HNF-4 regulated gene expression in the visceral endoderm: Tetraploid rescue of HNF-4-/- embryos. Development. 124 279-287 |
9. Signorini, S., Y. J. Liao, S. A. Duncan, L. Y. Jan and M. Stoffel (1997) Mice lacking G protein-coupled inwardly rectifying K+ channel GIRK2 are susceptible to seizures but have normal midbrain and cerebellum. Proc. Natl. Acad. Sci. USA. 94: 923-927 |
10. Molkentin, J . D., Q. Lin, S. A. Duncan, E. N. Olson (1997) Requirement of the transcription factor GATA4 for heart tube formation and ventral morphogenesis. Genes Dev. 11:1061-1072 |
11. Stoffel M. and S. A. Duncan (1997) The MODY1 transcription factor HNF-4a regulates expression of genes required for glucose transport and metabolism. Proc. Natl. Acad. Sci. USA 94, pp. 13209-13214. |
12. Rausa, F., H. Ye, L. Lim, S. Duncan and R. H. Costa. (1998). In Situ Hybridization with 33P Labeled RNA Probes for Determination of Cellular Expression Patterns of Liver Transcription Factors in Mouse Embryos. In Methods, A companion to Methods of Enzymology (ed. E. K. Wagner). Academic Press, San Diego, California. Volume 16, Issue 1. |
13. Duncan S. A., A. Navas, D. DuFort, J. Rossant, M. Stoffel (1998) Regulation of a transcription factor network required for differentiation and metabolism. Science 281: 609-740. |
14. Wilcock D., S. A. Duncan, P. Traktman, W-H. Zhang W. H. and G. L. Smith (1999). The vaccinia virus A40R gene product is a nonstructural, type II membrane glycoprotein that is expressed at the cell surface. J. Gen. Virol, 2137-48 |
15. Shih, D., Navas, M.A., Kuwajima, S., Duncan, S. A., Stoffel, M. (1999) Impaired glucose homeostasis and neonatal mortality in HNF-3a deficient mice. Proc Natl Acad Sci U S A. 31;96(18):10152-7 |
16. Li, J., Ning, G., S. A. Duncan (2000) Mammalian hepatocyte differentiation requires the transcription factor HNF-4a. Genes Dev 14:464-474. |
17. Haque J, Boger S, Li J, Duncan S. A. (2000) The murine pes1 gene encodes a nuclear protein containing a BRCT domain. Genomics. 70(2):201-10. |
18. Zhao R, Fahs SA, Weiler H, Duncan SA. (2001) An efficient method to successively introduce transgenes into a given genomic locus in the mouse. BMC Dev Biol 2001;1(1):10 Editor's Choice manuscript. |
19. Misra, R.P, Bronson, S.K., Xiao, Q., Garrison W., Li J., Zhao, R., Duncan SA. (2001) Generation of single-copy transgenic mouse embryos directly from ES cells by tetraploid embryo complementation BMC Biotechnology 2001 1:12 Editor's Choice manuscript. |
20. Parviz F, Li J, Kaestner KH, Duncan SA. Generation of a conditionally null allele of hnf4[alpha] Genesis 2002 Feb;32(2):130-133 |
21. 21 Misra R. P. and Duncan S.A. Gene targeting in the mouse: Advances in introduction of transgenes into the genome by homologous recombination. Endocrine 2002; 19 |
22. Lerch-Gaggl, A., J. Haque, J. Li, G. Ning, P. Traktman, and S. A. Duncan. Pescadillo is Essential for Nucleolar Assembly, Ribosome Biogenesis and Mammalian Cell Proliferation. J Biol Chem. 2002 Nov 22;277(47):45347-55 |
23. 23 Tirona RG, Lee W, Leake BF, Lan LB, Cline CB, Lamba V, Parviz F, Duncan SA, Inoue Y, Gonzalez FJ, Schuetz EG, Kim RB. The orphan nuclear receptor HNF4alpha determines PXR- and CAR-mediated xenobiotic induction of CYP3A4. Nat Med. 2003 Feb;9(2):220-4 |
24. Parviz F., Matullo C., Garrison W. D., Savatski L., Adamson J.W., Ning G., Kaestner K. H., Zaret, K.S. and Duncan SA. Hepatocyte nuclear factor 4a controls the development of a hepatic epithelium and liver morphogenesis. Nat Genet. 2003 Jul;34(3):292-6. |
25. Nelson TJ, Duncan SA, Misra RP. Conserved enhancer in the serum response factor promoter controls expression during early coronary vasculogenesis. Circ Res. 2004 Apr 30;94(8):1059-66 |
26. Lazarevich, N., Cheremnova, O., Varga, E., Ovchinnikov, D., Kudrjavtseva, E., Morozova, O., Fleishman O., Engelhardt N., and Duncan S.A. Hepatocarcinoma progression is associated with a downregulation in expression of hepatocyte nuclear factors. Hepatology 2004 Apr;39(4):1038-47. |
27. Watt A.J., Li J., and Duncan S.A. GATA4 is essential for formation of the proepicardium and regulates cardiogenesis. Proc Natl Acad Sci U S A. 2004 Aug 24;101(34):12573-8 |
28. Gupta RK, Vatamaniuk MZ, Lee CS, Flaschen RC, Fulmer JT, Matschinsky FM, Duncan SA, Kaestner KH. The MODY1 gene HNF-4alpha regulates selected genes involved in insulin secretion. J Clin Invest. 2005 |
29. Makita T, Duncan SA, Sucov HM. Retinoic acid, hypoxia, and GATA factors cooperatively control the onset of fetal liver erythropoietin expression and erythropoietic differentiation. Dev Biol. 2005 Apr 1;280(1):59-72. |
30. Zhao R, Watt AJ, Li J, Luebke-Wheeler J, Morrisey EE, Duncan SA. GATA6 Is Essential for Embryonic Development of the Liver but Dispensable for Early Heart Formation. Mol Cell Biol. 2005 Apr;25(7):2622-31. Cited by Faculty 1000, ASM journal highlight editorial. |
31. 31, Garrison W.D., Battle M.A, Yang C., Kaestner K.H., Sladek F.M., Duncan S.A. Hepatocyte Nuclear Facor 4α is essential for embryonic development of the mouse colon. Gastroenterology. 2006 Apr;130(4) |
32. Oka T, Maillet M., Watt, A.J., Schwartz, R.J., Aronow B.J., Duncan S.A., Molkentin JD., Cardiac-specific deletion of Gata4 reveals its requirement for hypertrophy, compensation, and myocyte viability. Circ Res. 2006 Mar 31:98(6):837-45 |
33. Sodhi CP., Li J. Duncan S.A. Generation of mice harbouring a conditional loss-of-function allele of Gata6 BMC Developmental Biology 2006, 6:19 |
34. Rufibach L.E., Duncan S.A., Battle M., Deeb S.S. Transcriptional regulation of the human hepatic lipase (LIPC) gene promoter. Transcriptional regulation of the human hepatic lipase (LIPC) gene promoter.J Lipid Res. 2006 Apr 7 |
35. Battle M.A., Konopka G., Parviz F. Lerch-Gaggl A., Yang C. Sladek F. Duncan S.A. HNF4 alpha orchestrates expression of cell adhesion proteins to coordinate the epithelial transformation of the developing liver 2006. Proc Natl Acad Sci U S A. 2006 May 30;103(22):8419-24. |
36. Xin M, Davis CA, Molkentin JD, Lien CL, Duncan SA, Richardson JA, Olson EN. A threshold of GATA4 and GATA6 expression is required for cardiovascular development. Proc Natl Acad Sci U S A. 2006 Jul 25;103(30):11189-94 |
37. Nelson, T.J., Ge Z., Van Orman J., Barron M., Rudy-Reil D., Hacker T.A., Duncan S.A., Misra R., Auchampach J.A.. Lough J. Improved cardiac function in infracted mice after treatment with pluripotent embryonic stem cells. Anat Rec A Discov Mol Cell Evol Biol. 2006 Nov;288(11):1216-24 |
38. Sirois J, Cote JF, Charest A, Uetani N, Bourdeau A, Duncan SA, Daniels E, Tremblay ML. Essential function of PTP-PEST during mouse embryonic vascularization, mesenchyme formation, neurogenesis and early liver development. Mech Dev. 2006 Dec;123(12):869-80. |
39. Lerch-Gaggl A., Sun K. Duncan S.A. Light Chain 1 of Microtubule-associated Protein 1B Can Negatively Regulate the Action of Pes1. J Biol Chem. 2007 Apr 13;282(15):11308-16. |
40. Watt AJ, Zhao R, Li J, Duncan SA. Development of the mammalian liver and ventral pancreas is dependent on GATA4. BMC Dev Biol. 2007 Apr 23;7(1):37 |
41. Konopka G., Tekiela j., Iverson M., Wells C., and Duncan S.A. Junctional adhesion molecule-A is critical for the formation of pseudocanaliculi and modulates E-cadherin expression in hepatic cells. J Biol Chem. 2007 Sep 21;282(38):28137-48. |
42. Cited2, a coactivator of HNF4alpha, is essential for liver development.
X Qu, E Lam, YQ Doughman, Y Chen, YT Chou, M Lam, M Turakhia, SL Dunwoodie, M Watanabe, B Xu, SA Duncan, and YC Yang
EMBO J. 2007; 26: 4445. |
43. Joerg Heineke, Mannix Auger-Messier, Jian Xu, Toru Oka, Michelle A. Sargent, Allen York, Raisa Klevitsky, Sachin Vaikunth, Stephen A. Duncan, Bruce J. Aronow, Jeffrey Robbins, Timothy M. Cromblehol, and Jeffery D. Molkentin Cardiomyocyte GATA4 functions as a stress-responsive regulator of angiogenesis in the murine heart J. Clin. Invest. 2007; 117:3198-3210. |
44. Zhao R, Watt AJ, Battle MA, Li J, Bondow BJ, Duncan SA. Loss of both GATA4 and GATA6 blocks cardiac myocyte differentiation and results in acardia in mice. Dev Biol. 2008 May 15;317(2):614-9 |
45. Nagaoka M, Hagiwara Y, Takemura K, Murakami, Y, Li J, Duncan SA, Akaiki T. Design of an artificial acellular feeder layer for the efficient propagation of mouse embryonic stem cells. J Biol Chem. 2008 Sep 26;283(39):26468-76 |
46. Luebke-Wheeler J, Zhang K, Battle M, Si-Tayeb K, Garrison W, Chhinder S, Li J, Kaufman RJ, Duncan SA. Hepatocyte nuclear factor 4alpha is implicated in endoplasmic reticulum stress-induced acute phase response by regulating expression of cyclic adenosine monophosphate responsive element binding protein H. Hepatology. 2008 May 30;48(4):1242-1250. |
47. Battle MA, Bondow BJ, Iverson MA, Adams SJ, Jandacek RJ, Tso P, Duncan SA GATA4 is essential for jejunal function in mice. Gastroenterology, 2008 Aug 3. |
48. Sun, K., Battle M.A., Misra R.P., Duncan S.A. Hepatocyte expression of Serum Response Factor is essential for liver function, hepoatocyte proliferation and survival, and postnatal body growth in mice. Hepatology. 2009 May;49(5):1645-54 |
49. Vecchi C., Montosi G., Zhang K., Lamberti I, Duncan S.A., Kaufman R.J., Pietrangelo A. ER Stress controls iron metabolism through induction of hepcidin. Science 2009 325(5942):877-80 |
50. Si-Tayeb, K, Fallon N., Nagaoki M., Ji J., Battle M.A., North P., Dalton S., Duncan S.A. Highly efficient generation of human hepatic cells from induced pluripotent stem cells Hepatology. 2010 Jan;51(1):297-305. |
51. Foxa1 functions as a pioneer transcription factor at transposable elements to activate Afp during differentiation of embryonic stem cells. Taube JH, Allton K, Duncan SA, Shen L, Barton MC. J Biol Chem. 2010 May 21;285(21):16135-44. |
52. Culture of human pluripotent stem cells using completely defined conditions on a recombinant E-cadherin substratum. Nagaoka M, Si-Tayeb K, Akaike T, Duncan SA. |
53. BMC Dev Biol. 2010 Jun 2;10(1):60. |
54. Si-Tayeb K, Noto FK, Sepac A, Sedlic F, Bosnjak ZJ, Lough JW, Duncan SA. Generation of human induced pluripotent stem cells by simple transient transfection of plasmid DNA encoding reprogramming factors. BMC Dev Biol. 2010 Aug 3;10:81. |
55. van Berlo JH, Elrod JW, van den Hoogenhof MM, York AJ, Aronow BJ, Duncan SA, Molkentin JD. The Transcription Factor GATA-6 Regulates Pathological Cardiac Hypertrophy. Circ Res. 2010 Aug 12. |
56. Sepac A, Sedlic F, Si-Tayeb K, Lough J, Duncan SA, Bienengraeber M, Park F, Kim J, Bosnjak ZJ. Isoflurane Preconditioning Elicits Competent Endogenous Mechanisms of Protection from Oxidative Stress in Cardiomyocytes Derived from Human Embryonic Stem Cells. Anesthesiology. 2010 Sep 3 |
57. Beuling E, Baffour-Awuah NY, Stapleton KA, Aronson BE, Noah TK, Shroyer NF, Duncan SA, Fleet JC, Krasinski SD. GATA Factors Regulate Proliferation, Differentiation, and Gene Expression in Small Intestine of Mature Mice. Gastroenterology. 2011 Jan 21. |
58. Van Orman JR, Si-Tayeb K, Duncan SA, Lough J. Induction of Cardiomyogenesis in Human Embryonic Stem Cells (hESCs) by hESC-Derived Definitive Endoderm. Stem Cells Dev. 2011 Jun 1. |
59. Lee JH, Giannikopoulos P, Duncan SA, Wang J, Johansen CT, Brown JD, Plutzky J, Hegele RA, Glimcher LH, Lee AH. Nat Med. The transcription factor cyclic AMP-responsive element-binding protein H regulates triglyceride metabolism. 2011 Jun 12;17(7):812-5. |
60. Ann DeLaForest, Masato Nagaoka, Karim Si-Tayeb, Fallon K. Noto, Genevieve Konopka, Michele A. Battle, and Stephen A. Duncan. HNF4A is essential for specification of hepatic progenitors from human pluripotent stem cells. Development. 2011 Oct;138(19):4143-53 |
61. Patankar, J. V., Chandak, P. G., Obrowsky, S., Pfeifer, T., Diwoky, C., Uellen, A., Sattler, W., Stollberger, R., Hoefler, G., Heinemann, A. et al. (2011). Loss of intestinal GATA4 prevents diet-induced obesity and promotes insulin sensitivity in mice. Am J Physiol Endocrinol Metab 300, E478-88. |
62. Zhang, C., Wang, G., Zheng, Z., Maddipati, K. R., Zhang, X., Dyson, G., Williams, P., Duncan, S. A., Kaufman, R. J. and Zhang, K. (2011). ER-tethered transcription factor crebh regulates hepatic lipogenesis, fatty acid oxidation, and lipolysis upon metabolic stress. Hepatology 2011 Nov 16. doi: 10.1002/hep.24783. |
63. Robert E. Schwartz, Kartik Trehan, Linda Andrus, Timothy P. Sheahan, Alexander Ploss, Stephen A. Duncan, Charles M. Rice, and Sangeeta N. Bhatia, Modeling hepatitis C virus infection using human induced pluripotent stem cells. Proc Natl Acad Sci U S A. 2012 Feb 14;109(7):2544-8 |
64. A Cell Surfaceome Map for Immunophenotyping and Sorting Pluripotent Stem Cells.
Gundry RL, Riordon DR, Tarasova Y, Chuppa S, Bhattacharya S, Juhasz O, Wiedemeier O, Milanovich S, Noto FK, Tchernyshyov I, Raginski K, Bausch-Fluck D, Tae HJ, Marshall S, Duncan SA, Wollscheid B, Wersto RP, Rao S, Van Eyk JE, Boheler KR.
Mol Cell Proteomics. 2012 Apr 6. |
65. Cayo MA, Cai J, Delaforest A, Noto FK, Nagaoka M, Clark BS, Collery RF, Si-Tayeb K, Duncan SA. 'JD' iPS cell-derived hepatocytes faithfully recapitulate the pathophysiology of familial hypercholesterolemia. Hepatology. 2012 May 31. Epub 2012 Sep 17. |
66. Cayo MA, Cai J, Delaforest A, Noto FK, Nagaoka M, Clark BS, Collery RF, Si-Tayeb K, Duncan SA. 'JD' iPS cell-derived hepatocytes faithfully recapitulate the pathophysiology of familial hypercholesterolemia. Hepatology. 2012 May 31. Epub 2012 Sep 17. |
67. Comparison of Cardiomyogenic Potential among Human ESC and iPSC Lines.
Sepac A, Si-Tayeb K, Sedlic F, Barrett S, Canfield S, Duncan SA, Bosnjak ZJ, Lough J.
Cell Transplant. 2012 Aug 2.
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68. Hepatocyte-like cells differentiated from human induced pluripotent stem cells: relevance to cellular therapies. Yu Y, Liu H, Ikeda Y, Amiot BP, Rinaldo P, Duncan SA, Nyberg SL. Stem Cell Res. 2012 Nov;9(3):196-207. |
69. Pancreas-specific deletion of mouse Gata4 and Gata6 causes pancreatic agenesis.
Xuan S, Borok MJ, Decker KJ, Battle MA, Duncan SA, Hale MA, Macdonald RJ, Sussel L. J Clin Invest. 2012 Oct 1;122(10):3516-28.
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70. Protocol for directed differentiation of human pluripotent stem cells toward a hepatocyte fate
Jun Cai, Ann DeLaForest, Joseph Fisher, Amanda Urick, Thomas Wagner, Kirk Twaroski, Max Cayo, Masato Nagaoka, Stephen A Duncan http://www.stembook.org/node/721
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Books, Chapters, and Reviews |
1. Frontiers in Diabetes v15. "Molecular Pathogenesis of MODYs" Ed. F.M. Matchinsky and M.A. Magnuson (2000) S.A. Duncan"Regulation of Gene Expression in the Visceral Endoderm by the MODY1 Transcription Factor HNF-4" |
2. Duncan SA. (2000) Transcriptional regulation of liver development. Dev Dyn.19(2):131-42. |
3. Duncan, S.A. (2001) The pancreas and its heartless beginnings. Nat Genet. 2001 Apr;27(4):355-6. |
4. Duncan, S.A. and A. Watt (2001) BMPs on the road to hepatogenesis. 2001 Genes Dev 15:1879-1884. |
5. Duncan, S.A. Mechanisms Controlling Early Development of The Liver. Mech Dev. 2003 Jan;120(1):19-33. |
6. 6 Watt A.J., Garrison W.D., Duncan S.A. Hnf4 alpha: a central regulator of hepatocyte differentiation and function. Hepatology 2003 Jun;37(6):1249-53. |
7. Zhao, R. and Duncan S. A. Embryonic development of the liver. Hepatology. 2005 May;41(5):956-967. |
8. Duncan S.A. Generation of embryos directly from embryonic stem cells by tetraploid embryo complementation reveals a role for GATA factors in organogenesis. Biochem Soc Trans. 2005 Dec;33(Pt 6):1534-6. |
9. Si-Tayeb, K., LeMaigre, F., and Duncan S.A. Organogenesis and Development of the Liver Si-Tayeb K, Lemaigre FP, Duncan SA. Dev Cell. 2010 Feb 16;18(2):175-89. |
10. Nagaoka M, Duncan SA. Transcriptional control of hepatocyte differentiation. Prog Mol Biol Transl Sci. 2010;97:79-101. |
11. Nagaoka, M. and Duncan, S. A. (2012). Laboratory-scale purification of a recombinant E-Cadherin-IgG Fc fusion protein that provides a cell surface matrix for extended culture and efficient subculture of human pluripotent stem cells. In Human embryonic and induced pluripotent stem cells: lineage specific differentiation protocols (ed. K. Ye and S. Jin), pp. 25-36. New York: Springer Science and Business Media, LLC. |
Editorials, Letters to Editor, Other |
1. Stephen A. Duncan, John W. Lough and Ravi Misra. Adult stem cells don't show same promise as embryonic stem cells. Milwaukee Journal Sentinel. Sept 7th 2006 |
2. Eye To Eye interview CBS 58 News April 05, 2009 Stem Cells - Dr. Stephen Duncan, Medical College of Wisconsin |
3. "Liver Laboratory" interview with Stephen Duncan. International Innovation, July 2011 pp 89-91. Available from (http://www.mcw.edu/FileLibrary/Groups/CellBioNeurobioAnatomy/images/Stephen_Duncan_InternationalIn.pdf) |
4. Stephen A. Duncan - The many benefits of fetal cell research. Op-Ed, Milwaukee Journal Sentinel, Sept 13th 2011 (http://www.jsonline.com/news/opinion/129764063.html). |
