Medical College of Wisconsin
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Charles R. Myers PhD

Professor

Institution: Medical College of Wisconsin
Department: Pharmacology and Toxicology
Program: Pharmacology

Member of the MCW Cancer Center


Research Experience

  • Acrolein
  • Chromium Compounds
  • Cytochromes b5
  • Cytochromes c
  • Environmental Exposure
  • Environmental Microbiology
  • Environmental Remediation
  • Iron
  • Manganese Compounds
  • Metals
  • Metals, Heavy
  • Occupational Exposure
  • Peroxiredoxins
  • Reactive Oxygen Species
  • Thioredoxin Reductase 1
  • Thioredoxin Reductase 2
  • Thioredoxins
  • Toxicology
  • Methodologies and Techniques

  • Blotting, Western
  • Cell Culture Techniques
  • Cloning, Molecular
  • Cytochromes b5
  • Endoplasmic Reticulum, Smooth
  • Membrane Potential, Mitochondrial
  • Membrane Proteins
  • Molecular Biology
  • Polymerase Chain Reaction
  • Spectrophotometry
  • Educational Expertise

  • Anti-Bacterial Agents
  • Antibiotics, Antifungal
  • Antibiotics, Antineoplastic
  • Antibiotics, Antitubercular
  • Cytochrome P-450 Enzyme System
  • Drug Resistance, Microbial
  • Pharmacogenetics
  • Pharmacology
  • Toxicology
  • Publications

  • Redox activation of Fe(III)-thiosemicarbazones and Fe(III)-bleomycin by thioredoxin reductase: specificity of enzymatic redox centers and analysis of reactive species formation by ESR spin trapping. (Myers JM, Cheng Q, Antholine WE, Kalyanaraman B, Filipovska A, Arnér ES, Myers CR) Free Radic Biol Med 2013 Jul;60:183-94 PMID 23485585
  • Redox activation of Fe(III)-thiosemicarbazones and Fe(III)-bleomycin by thioredoxin reductase: specificity of enzymatic redox centers and analysis of reactive species formation by ESR spin trapping (J. M. Myers, Q. Cheng, W. E. Antholine, B. Kalyanaraman, A. Filipovska, E. S. J. Arnér, C. R. Myers) Free Radic Biol Med DOI: 10.1016/j.freeradbiomed.2013.02.016
  • The effects of chromium(VI) on the thioredoxin system: implications for redox regulation. (Myers CR) Free Radic Biol Med 2012 May 15;52(10):2091-107 PMID 22542445
  • p38γ mitogen-activated protein kinase (MAPK) confers breast cancer hormone sensitivity by switching estrogen receptor (ER) signaling from classical to nonclassical pathway via stimulating ER phosphorylation and c-Jun transcription. (Qi X, Zhi H, Lepp A, Wang P, Huang J, Basir Z, Chitambar CR, Myers CR, Chen G) J Biol Chem 2012 Apr 27;287(18):14681-91 PMID 22399296
  • Coenzyme Q(1) as a probe for mitochondrial complex I activity in the intact perfused hyperoxia-exposed wild-type and Nqo1-null mouse lung. (Bongard RD, Myers CR, Lindemer BJ, Baumgardt S, Gonzalez FJ, Merker MP) Am J Physiol Lung Cell Mol Physiol 2012 May 1;302(9):L949-58 PMID 22268123
  • The effects of acrolein on the thioredoxin system: implications for redox-sensitive signaling. (Myers CR, Myers JM, Kufahl TD, Forbes R, Szadkowski A) Mol Nutr Food Res 2011 Sep;55(9):1361-74 PMID 21812108
  • The intracellular redox stress caused by hexavalent chromium is selective for proteins that have key roles in cell survival and thiol redox control. (Myers JM, Antholine WE, Myers CR) Toxicology 2011 Mar 15;281(1-3):37-47 PMID 21237240
  • The iron-chelating drug triapine causes pronounced mitochondrial thiol redox stress. (Myers JM, Antholine WE, Zielonka J, Myers CR) Toxicol Lett 2011 Mar 5;201(2):130-6 PMID 21195754
  • The pro-oxidant chromium(VI) inhibits mitochondrial complex I, complex II, and aconitase in the bronchial epithelium: EPR markers for Fe-S proteins. (Myers CR, Antholine WE, Myers JM) Free Radic Biol Med 2010 Dec 15;49(12):1903-15 PMID 20883776
  • The selenium-independent inherent pro-oxidant NADPH oxidase activity of mammalian thioredoxin reductase and its selenium-dependent direct peroxidase activities. (Cheng Q, Antholine WE, Myers JM, Kalyanaraman B, Arnér ES, Myers CR) J Biol Chem 2010 Jul 9;285(28):21708-23 PMID 20457604
  • The effects of hexavalent chromium on thioredoxin reductase and peroxiredoxins in human bronchial epithelial cells. (Myers JM, Myers CR) Free Radic Biol Med 2009 Nov 15;47(10):1477-85 PMID 19703554
  • The effects of acrolein on peroxiredoxins, thioredoxins, and thioredoxin reductase in human bronchial epithelial cells. (Myers CR, Myers JM) Toxicology 2009 Mar 4;257(1-2):95-104 PMID 19135121
  • Addition of DNA to Cr(VI) and cytochrome b5 containing proteoliposomes leads to generation of DNA strand breaks and Cr(III) complexes. (Borthiry GR, Antholine WE, Myers JM, Myers CR) Chem Biodivers 2008 Aug;5(8):1545-57 PMID 18729091
  • Hexavalent chromium causes the oxidation of thioredoxin in human bronchial epithelial cells. (Myers JM, Antholine WE, Myers CR) Toxicology 2008 Apr 18;246(2-3):222-33 PMID 18328613
  • Reductive activation of hexavalent chromium by human lung epithelial cells: generation of Cr(V) and Cr(V)-thiol species. (Borthiry GR, Antholine WE, Myers JM, Myers CR) J Inorg Biochem 2008 Jul;102(7):1449-62 PMID 18279960
  • Acrolein oxidizes the cytosolic and mitochondrial thioredoxins in human endothelial cells. (Szadkowski A, Myers CR) Toxicology 2008 Jan 14;243(1-2):164-76 PMID 18023956
  • Reduction of hexavalent chromium by human cytochrome b5: generation of hydroxyl radical and superoxide. (Borthiry GR, Antholine WE, Kalyanaraman B, Myers JM, Myers CR) Free Radic Biol Med 2007 Mar 15;42(6):738-55; discussion 735-7 PMID 17320757
  • The outer membrane cytochromes of Shewanella oneidensis MR-1 are lipoproteins. (Myers CR, Myers JM) Lett Appl Microbiol 2004;39(5):466-70 PMID 15482439
  • Shewanella oneidensis MR-1 restores menaquinone synthesis to a menaquinone-negative mutant. (Myers CR, Myers JM) Appl Environ Microbiol 2004 Sep;70(9):5415-25 PMID 15345428
  • The outer membrane protein Omp35 affects the reduction of Fe(III), nitrate, and fumarate by Shewanella oneidensis MR-1. (Maier TM, Myers CR) BMC Microbiol 2004 Jun 22;4:23 PMID 15212692
  • Vanadium(V) reduction by Shewanella oneidensis MR-1 requires menaquinone and cytochromes from the cytoplasmic and outer membranes. (Myers JM, Antholine WE, Myers CR) Appl Environ Microbiol 2004 Mar;70(3):1405-12 PMID 15006760
  • Cell surface exposure of the outer membrane cytochromes of Shewanella oneidensis MR-1. (Myers CR, Myers JM) Lett Appl Microbiol 2003;37(3):254-8 PMID 12904229
  • Identification of the gene encoding the sole physiological fumarate reductase in Shewanella oneidensis MR-1. (Maier TM, Myers JM, Myers CR) J Basic Microbiol 2003;43(4):312-27 PMID 12872312
  • Overlapping role of the outer membrane cytochromes of Shewanella oneidensis MR-1 in the reduction of manganese(IV) oxide. (Myers JM, Myers CR) Lett Appl Microbiol 2003;37(1):21-5 PMID 12803550
  • MtrB is required for proper incorporation of the cytochromes OmcA and OmcB into the outer membrane of Shewanella putrefaciens MR-1. (Myers CR, Myers JM) Appl Environ Microbiol 2002 Nov;68(11):5585-94 PMID 12406753
  • Evaluating molar CYP1A level in fish hepatic microsomes by competitive ELISA using recombinant membrane-free CYP1A standard protein. (Tom M, Myers CR, Waterman MR) Aquat Toxicol 2002 Sep 10;59(1-2):101-14 PMID 12088637
  • Genetic complementation of an outer membrane cytochrome omcB mutant of Shewanella putrefaciens MR-1 requires omcB plus downstream DNA. (Myers JM, Myers CR) Appl Environ Microbiol 2002 Jun;68(6):2781-93 PMID 12039733
  • Isolation and characterization of a Shewanella putrefaciens MR-1 electron transport regulator etrA mutant: reassessment of the role of EtrA. (Maier TM, Myers CR) J Bacteriol 2001 Aug;183(16):4918-26 PMID 11466298
  • Cytochrome b(5) plays a key role in human microsomal chromium(VI) reduction. (Jannetto PJ, Antholine WE, Myers CR) Toxicology 2001 Feb 28;159(3):119-33 PMID 11223168
  • Role for outer membrane cytochromes OmcA and OmcB of Shewanella putrefaciens MR-1 in reduction of manganese dioxide. (Myers JM, Myers CR) Appl Environ Microbiol 2001 Jan;67(1):260-9 PMID 11133454
  • Chromium(VI) reductase activity is associated with the cytoplasmic membrane of anaerobically grown Shewanella putrefaciens MR-1. (Myers CR, Carstens BP, Antholine WE, Myers JM) J Appl Microbiol 2000 Jan;88(1):98-106 PMID 10735248
  • Role of the tetraheme cytochrome CymA in anaerobic electron transport in cells of Shewanella putrefaciens MR-1 with normal levels of menaquinone. (Myers JM, Myers CR) J Bacteriol 2000 Jan;182(1):67-75 PMID 10613864
  • Nitric oxide inhibits aldosterone synthesis by a guanylyl cyclase-independent effect. (Hanke CJ, Drewett JG, Myers CR, Campbell WB) Endocrinology 1998 Oct;139(10):4053-60 PMID 9751482
  • Isolation and sequence of omcA, a gene encoding a decaheme outer membrane cytochrome c of Shewanella putrefaciens MR-1, and detection of omcA homologs in other strains of S. putrefaciens. (Myers JM, Myers CR) Biochim Biophys Acta 1998 Aug 14;1373(1):237-51 PMID 9733973
  • Iron stimulates the rate of reduction of hexavalent chromium by human microsomes. (Myers CR, Myers JM) Carcinogenesis 1998 Jun;19(6):1029-38 PMID 9667741
  • Isolation and characterization of a transposon mutant of Shewanella putrefaciens MR-1 deficient in fumarate reductase. (Myers CR, Myers JM) Lett Appl Microbiol 1997 Sep;25(3):162-8 PMID 9351256
  • Outer membrane cytochromes of Shewanella putrefaciens MR-1: spectral analysis, and purification of the 83-kDa c-type cytochrome. (Myers CR, Myers JM) Biochim Biophys Acta 1997 Jun 12;1326(2):307-18 PMID 9218561
  • Antibodies to a synthetic peptide that react with flavin-containing monooxygenase (HLFMO3) in human hepatic microsomes. (Myers CR, Porgilsson B, Myers JM) J Pharmacol Toxicol Methods 1997 Mar;37(2):61-6 PMID 9174980
  • Replication of plasmids with the p15A origin in Shewanella putrefaciens MR-1. (Myers CR, Myers JM) Lett Appl Microbiol 1997 Mar;24(3):221-5 PMID 9080705
  • Cloning and sequence of cymA, a gene encoding a tetraheme cytochrome c required for reduction of iron(III), fumarate, and nitrate by Shewanella putrefaciens MR-1. (Myers CR, Myers JM) J Bacteriol 1997 Feb;179(4):1143-52 PMID 9023196
  • Ferric iron reduction-linked growth yields of Shewanella putrefaciens MR-1. (Myers CR, Myers JM) J Appl Bacteriol 1994 Mar;76(3):253-8 PMID 8157545
  • Enzymatic reduction of chromium(VI) by human hepatic microsomes. (Pratt PF, Myers CR) Carcinogenesis 1993 Oct;14(10):2051-7 PMID 8222053
  • Localization of cytochromes to the outer membrane of anaerobically grown Shewanella putrefaciens MR-1. (Myers CR, Myers JM) J Bacteriol 1992 Jun;174(11):3429-38 PMID 1592800
  • Microbial reduction of manganese and iron: new approaches to carbon cycling. (Nealson KH, Myers CR) Appl Environ Microbiol 1992 Feb;58(2):439-43 PMID 1610166
  • Respiration-linked proton translocation coupled to anaerobic reduction of manganese(IV) and iron(III) in Shewanella putrefaciens MR-1. (Myers CR, Nealson KH) J Bacteriol 1990 Nov;172(11):6232-8 PMID 2172208
  • Bacterial manganese reduction and growth with manganese oxide as the sole electron acceptor. (Myers CR, Nealson KH) Science 1988 Jun 3;240(4857):1319-21 PMID 17815852
  • Cell-cycle-specific fluctuation in cytoplasmic membrane composition in aerobically grown Rhodospirillum rubrum. (Myers CR, Collins ML) J Bacteriol 1987 Dec;169(12):5445-51 PMID 3119564
  • Cell-cycle-specific oscillation in the composition of chromatophore membrane in Rhodospirillum rubrum. (Myers CR, Collins ML) J Bacteriol 1986 Jun;166(3):818-23 PMID 3086290
  • Post-embedding direct immunogold detection of a protein antigen in insect tissue. (Myers CR, Bergtrom G, Crook SM, Boyer DR, Collins ML) J Histochem Cytochem 1986 Feb;34(2):221-6 PMID 3511140
  • Grants and Awards

  • Human Lung Chromium Toxicity: Role of cytochrome b5 (#9203485 09/27/2004 - 07/31/2009) Sponsor: NIH Environmental Health Sciences
  • Role of Outer Membrane Cytochromes in Metal Reduction (#9202968 08/01/2003 - 07/31/2008) Sponsor: National Science Foundation
  • Development of Redox Biology Program at the Medical College of Wisconsin (#9520214 11/01/2011 - 06/30/2017) PI of Coordinated Disruption of Peroxiredoxin Function: Novel Antitumor Drug Combinations (#5520268)