Medical College of Wisconsin
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Debra K. Newman PhD

Debra K. Newman PhD

Investigator

Institution: BloodCenter of Wisconsin
Department: Blood Research Institute

Member of the MCW Cancer Center


Research Experience

  • Antigens, CD31
  • Blood Platelets
  • Phosphotyrosine
  • Platelet Activation
  • Platelet Adhesiveness
  • Platelet Aggregation
  • Platelet Membrane Glycoproteins
  • Protein Interaction Domains and Motifs
  • Protein Tyrosine Phosphatases
  • Signal Transduction
  • Publications

  • B-cell tolerance regulates production of antibodies causing heparin-induced thrombocytopenia. (Zheng Y, Wang AW, Yu M, Padmanabhan A, Tourdot BE, Newman DK, White GC, Aster RH, Wen R, Wang D) Blood 2014 Feb 6;123(6):931-4 PMID 24357731
  • Critical role for mouse marginal zone B cells in PF4/heparin antibody production. (Zheng Y, Yu M, Podd A, Yuan L, Newman DK, Wen R, Arepally G, Wang D) Blood 2013 Apr 25;121(17):3484-92 PMID 23460609
  • Immunoreceptor tyrosine-based inhibitory motif (ITIM)-mediated inhibitory signaling is regulated by sequential phosphorylation mediated by distinct nonreceptor tyrosine kinases: a case study involving PECAM-1. (Tourdot BE, Brenner MK, Keough KC, Holyst T, Newman PJ, Newman DK) Biochemistry 2013 Apr 16;52(15):2597-608 PMID 23418871
  • Cooperative integrin/ITAM signaling in platelets enhances thrombus formation in vitro and in vivo. (Zhi H, Rauova L, Hayes V, Gao C, Boylan B, Newman DK, McKenzie SE, Cooley BC, Poncz M, Newman PJ) Blood 2013 Mar 7;121(10):1858-67 PMID 23264598
  • Platelet signaling. (Stalker TJ, Newman DK, Ma P, Wannemacher KM, Brass LF) Handb Exp Pharmacol 2012(210):59-85 PMID 22918727
  • Cadherin 6 has a functional role in platelet aggregation and thrombus formation. (Dunne E, Spring CM, Reheman A, Jin W, Berndt MC, Newman DK, Newman PJ, Ni H, Kenny D) Arterioscler Thromb Vasc Biol 2012 Jul;32(7):1724-31 PMID 22539596
  • A newly identified complex of spinophilin and the tyrosine phosphatase, SHP-1, modulates platelet activation by regulating G protein-dependent signaling. (Ma P, Cierniewska A, Signarvic R, Cieslak M, Kong H, Sinnamon AJ, Neubig RR, Newman DK, Stalker TJ, Brass LF) Blood 2012 Feb 23;119(8):1935-45 PMID 22210881
  • PECAM-1 dampens cytokine levels during LPS-induced endotoxemia by regulating leukocyte trafficking. (Privratsky JR, Tilkens SB, Newman DK, Newman PJ) Life Sci 2012 Jan 30;90(5-6):177-84 PMID 22119535
  • Relative contribution of PECAM-1 adhesion and signaling to the maintenance of vascular integrity. (Privratsky JR, Paddock CM, Florey O, Newman DK, Muller WA, Newman PJ) J Cell Sci 2011 May 1;124(Pt 9):1477-85 PMID 21486942
  • Residues within a lipid-associated segment of the PECAM-1 cytoplasmic domain are susceptible to inducible, sequential phosphorylation. (Paddock C, Lytle BL, Peterson FC, Holyst T, Newman PJ, Volkman BF, Newman DK) Blood 2011 Jun 2;117(22):6012-23 PMID 21464369
  • Heparin promotes platelet responsiveness by potentiating αIIbβ3-mediated outside-in signaling. (Gao C, Boylan B, Fang J, Wilcox DA, Newman DK, Newman PJ) Blood 2011 May 5;117(18):4946-52 PMID 21368290
  • Lyn and PECAM-1 function as interdependent inhibitors of platelet aggregation. (Ming Z, Hu Y, Xiang J, Polewski P, Newman PJ, Newman DK) Blood 2011 Apr 7;117(14):3903-6 PMID 21297004
  • Platelet-endothelial cell adhesion molecule-1 regulates endothelial NO synthase activity and localization through signal transducers and activators of transcription 3-dependent NOSTRIN expression. (McCormick ME, Goel R, Fulton D, Oess S, Newman D, Tzima E) Arterioscler Thromb Vasc Biol 2011 Mar;31(3):643-9 PMID 21183735
  • Mechanism of outside-in {alpha}IIb{beta}3-mediated activation of human platelets by the colonizing Bacterium, Streptococcus gordonii. (Keane C, Petersen H, Reynolds K, Newman DK, Cox D, Jenkinson HF, Newman PJ, Kerrigan SW) Arterioscler Thromb Vasc Biol 2010 Dec;30(12):2408-15 PMID 21071690
  • Platelet endothelial cell adhesion molecule-1 regulates collagen-stimulated platelet function by modulating the association of phosphatidylinositol 3-kinase with Grb-2-associated binding protein-1 and linker for activation of T cells. (Moraes LA, Barrett NE, Jones CI, Holbrook LM, Spyridon M, Sage T, Newman DK, Gibbins JM) J Thromb Haemost 2010 Nov;8(11):2530-41 PMID 20723025
  • PECAM-1: conflicts of interest in inflammation. (Privratsky JR, Newman DK, Newman PJ) Life Sci 2010 Jul 17;87(3-4):69-82 PMID 20541560
  • PECAM-1 functions as a negative regulator of laminin-induced platelet activation. (Crockett J, Newman DK, Newman PJ) J Thromb Haemost 2010 Jul;8(7):1584-93 PMID 20403098
  • TCR stimulation drives cleavage and shedding of the ITIM receptor CD31. (Fornasa G, Groyer E, Clement M, Dimitrov J, Compain C, Gaston AT, Varthaman A, Khallou-Laschet J, Newman DK, Graff-Dubois S, Nicoletti A, Caligiuri G) J Immunol 2010 May 15;184(10):5485-92 PMID 20400708
  • Neutrophil transmigration mediated by the neutrophil-specific antigen CD177 is influenced by the endothelial S536N dimorphism of platelet endothelial cell adhesion molecule-1. (Bayat B, Werth S, Sachs UJ, Newman DK, Newman PJ, Santoso S) J Immunol 2010 Apr 1;184(7):3889-96 PMID 20194726
  • The anti-inflammatory actions of platelet endothelial cell adhesion molecule-1 do not involve regulation of endothelial cell NF-kappa B. (Privratsky JR, Tourdot BE, Newman DK, Newman PJ) J Immunol 2010 Mar 15;184(6):3157-63 PMID 20173029
  • PI3Kbeta goes to the head of its class. (Newman DK) Blood 2009 Sep 3;114(10):2011-2 PMID 19729533
  • The Y's that bind: negative regulators of Src family kinase activity in platelets. (Newman DK) J Thromb Haemost 2009 Jul;7 Suppl 1:195-9 PMID 19630799
  • Eptifibatide-induced thrombocytopenia and thrombosis in humans require FcgammaRIIa and the integrin beta3 cytoplasmic domain. (Gao C, Boylan B, Bougie D, Gill JC, Birenbaum J, Newman DK, Aster RH, Newman PJ) J Clin Invest 2009 Mar;119(3):504-11 PMID 19197137
  • Site-specific effects of PECAM-1 on atherosclerosis in LDL receptor-deficient mice. (Goel R, Schrank BR, Arora S, Boylan B, Fleming B, Miura H, Newman PJ, Molthen RC, Newman DK) Arterioscler Thromb Vasc Biol 2008 Nov;28(11):1996-2002 PMID 18669884
  • Identification of FcgammaRIIa as the ITAM-bearing receptor mediating alphaIIbbeta3 outside-in integrin signaling in human platelets. (Boylan B, Gao C, Rathore V, Gill JC, Newman DK, Newman PJ) Blood 2008 Oct 1;112(7):2780-6 PMID 18641368
  • An alternatively spliced isoform of PECAM-1 is expressed at high levels in human and murine tissues, and suggests a novel role for the C-terminus of PECAM-1 in cytoprotective signaling. (Bergom C, Paddock C, Gao C, Holyst T, Newman DK, Newman PJ) J Cell Sci 2008 Apr 15;121(Pt 8):1235-42 PMID 18388311
  • The proinflammatory phenotype of PECAM-1-deficient mice results in atherogenic diet-induced steatohepatitis. (Goel R, Boylan B, Gruman L, Newman PJ, North PE, Newman DK) Am J Physiol Gastrointest Liver Physiol 2007 Dec;293(6):G1205-14 PMID 17932230
  • High-throughput phosphotyrosine profiling using SH2 domains. (Machida K, Thompson CM, Dierck K, Jablonowski K, Kärkkäinen S, Liu B, Zhang H, Nash PD, Newman DK, Nollau P, Pawson T, Renkema GH, Saksela K, Schiller MR, Shin DG, Mayer BJ) Mol Cell 2007 Jun 22;26(6):899-915 PMID 17588523
  • Paxillin family members function as Csk-binding proteins that regulate Lyn activity in human and murine platelets. (Rathore VB, Okada M, Newman PJ, Newman DK) Biochem J 2007 Apr 15;403(2):275-81 PMID 17233630
  • The cell-adhesion and signaling molecule PECAM-1 is a molecular mediator of resistance to genotoxic chemotherapy. (Bergom C, Goel R, Paddock C, Gao C, Newman DK, Matsuyama S, Newman PJ) Cancer Biol Ther 2006 Dec;5(12):1699-707 PMID 17106245
  • The alleles of PECAM-1. (Novinska MS, Pietz BC, Ellis TM, Newman DK, Newman PJ) Gene 2006 Jul 5;376(1):95-101 PMID 16581204
  • Platelet PECAM-1 inhibits thrombus formation in vivo. (Falati S, Patil S, Gross PL, Stapleton M, Merrill-Skoloff G, Barrett NE, Pixton KL, Weiler H, Cooley B, Newman DK, Newman PJ, Furie BC, Furie B, Gibbins JM) Blood 2006 Jan 15;107(2):535-41 PMID 16166583
  • Peroxynitrite reduces the endothelium-derived hyperpolarizing factor component of coronary flow-mediated dilation in PECAM-1-knockout mice. (Liu Y, Bubolz AH, Shi Y, Newman PJ, Newman DK, Gutterman DD) Am J Physiol Regul Integr Comp Physiol 2006 Jan;290(1):R57-65 PMID 16166207
  • An important role of phospholipase Cgamma1 in pre-B-cell development and allelic exclusion. (Wen R, Chen Y, Schuman J, Fu G, Yang S, Zhang W, Newman DK, Wang D) EMBO J 2004 Oct 13;23(20):4007-17 PMID 15372077
  • Phospholipase Cgamma2 contributes to stable thrombus formation on VWF. (Rathore V, Wang D, Newman DK, Newman PJ) FEBS Lett 2004 Aug 27;573(1-3):26-30 PMID 15327970
  • Endothelial cell PECAM-1 confers protection against endotoxic shock. (Maas M, Stapleton M, Bergom C, Mattson DL, Newman DK, Newman PJ) Am J Physiol Heart Circ Physiol 2005 Jan;288(1):H159-64 PMID 15319204
  • Anti-GPVI-associated ITP: an acquired platelet disorder caused by autoantibody-mediated clearance of the GPVI/FcRgamma-chain complex from the human platelet surface. (Boylan B, Chen H, Rathore V, Paddock C, Salacz M, Friedman KD, Curtis BR, Stapleton M, Newman DK, Kahn ML, Newman PJ) Blood 2004 Sep 1;104(5):1350-5 PMID 15150079
  • PECAM-1 negatively regulates GPIb/V/IX signaling in murine platelets. (Rathore V, Stapleton MA, Hillery CA, Montgomery RR, Nichols TC, Merricks EP, Newman DK, Newman PJ) Blood 2003 Nov 15;102(10):3658-64 PMID 12893757
  • Reactive oxygen species induce reversible PECAM-1 tyrosine phosphorylation and SHP-2 binding. (Maas M, Wang R, Paddock C, Kotamraju S, Kalyanaraman B, Newman PJ, Newman DK) Am J Physiol Heart Circ Physiol 2003 Dec;285(6):H2336-44 PMID 12893640
  • Signal transduction pathways mediated by PECAM-1: new roles for an old molecule in platelet and vascular cell biology. (Newman PJ, Newman DK) Arterioscler Thromb Vasc Biol 2003 Jun 1;23(6):953-64 PMID 12689916
  • PECAM-1 functions as a specific and potent inhibitor of mitochondrial-dependent apoptosis. (Gao C, Sun W, Christofidou-Solomidou M, Sawada M, Newman DK, Bergom C, Albelda SM, Matsuyama S, Newman PJ) Blood 2003 Jul 1;102(1):169-79 PMID 12649141
  • Nitration of PECAM-1 ITIM tyrosines abrogates phosphorylation and SHP-2 binding. (Newman DK, Hoffman S, Kotamraju S, Zhao T, Wakim B, Kalyanaraman B, Newman PJ) Biochem Biophys Res Commun 2002 Sep 6;296(5):1171-9 PMID 12207897
  • Inhibition of antigen-receptor signaling by Platelet Endothelial Cell Adhesion Molecule-1 (CD31) requires functional ITIMs, SHP-2, and p56(lck). (Newman DK, Hamilton C, Newman PJ) Blood 2001 Apr 15;97(8):2351-7 PMID 11290597
  • Platelet endothelial cell adhesion molecule-1 serves as an inhibitory receptor that modulates platelet responses to collagen. (Patil S, Newman DK, Newman PJ) Blood 2001 Mar 15;97(6):1727-32 PMID 11238114
  • Invariant chains with the class II binding site replaced by a sequence from influenza virus matrix protein constrain low-affinity sequences to MHC II presentation. (Carstens C, Newman DK, Bohlen H, König A, Koch N) Int Immunol 2000 Nov;12(11):1561-8 PMID 11058576
  • A new role for platelet-endothelial cell adhesion molecule-1 (CD31): inhibition of TCR-mediated signal transduction. (Newton-Nash DK, Newman PJ) J Immunol 1999 Jul 15;163(2):682-8 PMID 10395658
  • Interferon-gamma secretion defects in haemophilia A patients receiving highly purified plasma-derived or recombinant factor VIII. (Newton-Nash DK, Tollerud D, Guevarra L, Gill JC) Br J Haematol 1996 Dec;95(3):554-60 PMID 8943901
  • T cell recognition of MHC class II-associated peptides is independent of peptide affinity for MHC and sodium dodecyl sulfate stability of the peptide/MHC complex. Effects of conservative amino acid substitutions at anchor position 1 of influenza matrix protein19-31. (Wu S, Gorski J, Eckels DD, Newton-Nash DK) J Immunol 1996 May 15;156(10):3815-20 PMID 8621918
  • Bacterial toxin superantigens stimulate all members of susceptible VB gene families. (Yassai M, Newton-Nash D, Gorski J) Ann N Y Acad Sci 1995 Jul 7;756:110-2 PMID 7645813
  • Production and characterization of transformed B-lymphocytes expressing the membrane defect of Scott syndrome. (Kojima H, Newton-Nash D, Weiss HJ, Zhao J, Sims PJ, Wiedmer T) J Clin Invest 1994 Dec;94(6):2237-44 PMID 7989579
  • The molecular basis of allorecognition. Assessment of the involvement of peptide. (Newton-Nash DK) Hum Immunol 1994 Oct;41(2):105-11 PMID 7860354
  • Differential effect of polymorphism at HLA-DR1 beta-chain positions 85 and 86 on binding and recognition of DR1-restricted antigenic peptides. (Newton-Nash DK, Eckels DD) J Immunol 1993 Mar 1;150(5):1813-21 PMID 7679697
  • Effects of localized HLA class II beta chain polymorphism on binding of antigenic peptide and stimulation of T cells. (Newton-Nash DK, Eckels DD) Hum Immunol 1992 Mar;33(3):213-23 PMID 1618659
  • T cell receptor and alloimmune thrombocytopenias: a model for autoimmune diseases? (Yassai M, McFarland JG, Newton-Nash D, Newman PJ, Eckels DD, Gorski J) Ann Med Interne (Paris) 1992;143(6):365-70 PMID 1295386
  • Assessment of chemokinetic behavior of inflammatory lung macrophages in a linear under-agarose assay. (Newton-Nash DK, Tonellato P, Swiersz M, Abramoff P) J Leukoc Biol 1990 Oct;48(4):297-305 PMID 2394943
  • Pathogenesis of acute pulmonary inflammation in strain 2 and strain 13 guinea pigs. (Newton-Nash DK, Pontzer CH, Kahn L, Garancis JC, Abramoff P) Reg Immunol 1988 Sep-Oct;1(2):111-8 PMID 3275213
  • Cellular immunoregulation of acute pulmonary inflammation in strain 2 guinea pigs. (Pontzer CH, Newton-Nash DK, Garancis JC, Abramoff P) Reg Immunol 1988 Sep-Oct;1(2):119-27 PMID 2978946
  • Monoamine oxidase types A and B in the vertebrate brain. (Hall TR, Yurgens PB, Figueroa HR, Urueña G, Olcese JM, Newton DK, Vorwald SR) Comp Biochem Physiol C 1982;71C(1):107-10 PMID 6121664
  • Failure of exogenous serotonin to inhibit the release of 3H-serotonin induced by electrical field stimulation in superfused goldfish brain slices. (Figueroa HR, Newton DK, Yurgens PB, Olcese JM, Hall TR) Gen Pharmacol 1982;13(2):157-60 PMID 7095394