Faculty Collaboration Database

Localization of arginine biosynthetic enzymes in renal proximal tubules and abundance of mRNA during development. Pediatr Res 1991 Feb;29(2):151-4

Date

02/01/1991

Pubmed ID

2014150

DOI

10.1203/00006450-199102000-00010

Scopus ID

2-s2.0-0025963628 (requires institutional sign-in at Scopus site)   30 Citations

Abstract

Argininosuccinate synthetase and argininosuccinate lyase catalyze the conversion of citrulline to arginine in kidney. Immunohistochemical staining of mouse kidney sections with antibodies to these two enzymes, compared with the staining patterns of known markers for proximal tubules, demonstrated that these enzymes are localized within the proximal tubules. The relative abundance of mRNA encoding argininosuccinate synthetase and argininosuccinate lyase during fetal and postnatal development of mouse kidney was also determined. Changes in relative abundance of these mRNA in kidney are coordinate during development, paralleling the developmental profile of phosphoenolpyruvate carboxykinase mRNA, which is also expressed in proximal tubules. Although relative abundances of the mRNA are comparable in liver and kidney of adult mice, the profiles of mRNA abundance during development of these two organs are distinct. The results indicate that these enzymes and their corresponding mRNA can serve as useful markers for examining the differentiation and development of renal proximal tubules in vivo and in cultured explants.

Author List

Morris SM Jr, Sweeney WE Jr, Kepka DM, O'Brien WE, Avner ED

Author

Ellis D. Avner MD Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Arginine
Argininosuccinate Lyase
Argininosuccinate Synthase
Female
Fetus
Gene Expression
Immunohistochemistry
Kidney Tubules, Proximal
Mice
Pregnancy
RNA, Messenger