Faculty Collaboration Database

Construction of a gene library from the nitrogen-fixing aerobe Azotobacter vinelandii. Gene 1983 Nov;25(2-3):355-60

Date

11/01/1983

Pubmed ID

6319242

DOI

10.1016/0378-1119(83)90240-8

Scopus ID

2-s2.0-0021048908 (requires institutional sign-in at Scopus site)   6 Citations

Abstract

We have cloned the DNA of Azotobacter vinelandii in the cosmid pHC79. Recombinant cosmids that can transform Escherichia coli leuB- to a Leu+ phenotype, as well as those having sequence homology to the nitrogenase structural genes of Klebsiella pneumoniae have been selected from this library.

Author List

Medhora M, Phadnis SH, Das HK

MESH terms used to index this publication - Major topics in bold

Azotobacter
Culture Media
DNA Restriction Enzymes
DNA, Bacterial
Genes, Bacterial
Leucine
Nitrogenase
Plasmids
Transduction, Genetic
Transformation, Genetic