Faculty Collaboration Database

Adoptive transfer of acute lung injury. Am J Physiol Lung Cell Mol Physiol 2000 Nov;279(5):L985-93

Date

10/29/2000

Pubmed ID

11053036

DOI

10.1152/ajplung.2000.279.5.L985

Scopus ID

2-s2.0-0033696812 (requires institutional sign-in at Scopus site)   14 Citations

Abstract

In this study, we describe a novel adoptive transfer protocol to study acute lung injury in the rat. We show that bronchoalveolar lavage (BAL) cells isolated from rats 5 h after intratracheal administration of lipopolysaccharide (LPS) induce a lung injury when transferred to normal control recipient rats. This lung injury is characterized by increased alveolar-arterial oxygen difference and extravasation of Evans blue dye (EBD) into lungs of recipient rats. Recipient rats receiving similar numbers of donor cells isolated from healthy rats do not show adverse changes in the alveolar-arterial oxygen difference or in extravasation of EBD. The adoptive transfer-induced lung injury is associated with increased numbers of neutrophils in the BAL, the levels of which are similar to the numbers observed in BAL cells isolated from rats treated for 5 h with LPS. As an indicator of BAL cell activation, donor BAL cell inducible nitric oxide synthase (iNOS) expression was compared with BAL cell iNOS expression 48 h after adoptive transfer. BAL cells isolated 5 h after LPS administration expressed iNOS immediately after isolation. In contrast, BAL cells isolated 48 h after adoptive transfer did not express iNOS immediately after isolation but expressed iNOS following a 24-h ex vivo culture. These findings indicate that the activation state of donor BAL cells differs from BAL cells isolated 48 h after adoptive transfer, suggesting that donor BAL cells may stimulate migration of new inflammatory cells into the recipient rats lungs.

Author List

Moxley MA, Baird TL, Corbett JA

Author

John A. Corbett PhD Chair, Professor in the Biochemistry department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adoptive Transfer
Animals
Bronchoalveolar Lavage Fluid
Cell Line
Cell Transplantation
Eosinophils
Gene Expression Regulation, Enzymologic
Lipopolysaccharides
Lung
Lung Injury
Macrophages, Alveolar
Male
Mice
Neutrophils
Nitric Oxide Synthase
Nitric Oxide Synthase Type II
Rats
Rats, Sprague-Dawley