A human parathyroid carcinoma that produces parathyroid hormone: long term maintenance in tissue culture. J Clin Endocrinol Metab 1981 Sep;53(3):641-4
Date
09/01/1981Pubmed ID
7263844DOI
10.1210/jcem-53-3-641Scopus ID
2-s2.0-0019718515 (requires institutional sign-in at Scopus site) 10 CitationsAbstract
Parathyroid carcinoma cells from a pulmonary metastasis of a patient with a serum Ca of 17 mg/dl and an immunoreactive parathyroid hormone (PTH) of 6.4 ng eq bovine (b) PTH/ml (normal 40--400 pg/ml) have been maintained in tissue culture for more than 2 1/2 years. The cells secrete PTH into the culture media that 1) during immunoassay dilutes in parallel to human hyperparathyroid serum, 2) has a molecular weight similar to intact highly purified bPTH, and 3) stimulates bone resorption in a manner that is equivalent and additive to synthetic bPTH-(1--34).
Author List
Pattillo RA, Ruckert AC, Wilson SD, Hussa RO, Gray RW, Lemann J JrMESH terms used to index this publication - Major topics in bold
AdultBiological Assay
Bone Resorption
Calcium
Culture Techniques
Female
Humans
Lung Neoplasms
Parathyroid Hormone
Parathyroid Neoplasms