Faculty Collaboration Database

Nitric oxide production by the rat insulinoma cell line, RINm5F, Is specific for IL-1: a spectrophotometric IL-1 bioassay. Anal Biochem 1996 Apr 05;236(1):14-9

Date

04/05/1996

Pubmed ID

8619479

DOI

10.1006/abio.1996.0125

Scopus ID

2-s2.0-0029875376 (requires institutional sign-in at Scopus site)   18 Citations

Abstract

Cytokines inhibit glucose-induced insulin secretion from pancreatic beta-cells by stimulating the expression of nitric oxide synthase and the increased production of nitric oxide (NO). We have found that the rat insulinoma cell line, RINm5F, responds specifically and linearly to murine and human interleukin-1beta (IL-1beta) and IL-1alpha in the range of 0.1 to 1 unit/ml to produce nitric oxide. Other cytokines, including IL-2, IL-4, IL-6, IL-9, IL-11, IL-15, tumor necrosis factor-alpha, interferon-gamma, and lipopolysaccharide fail to stimulate nitric oxide formation by RINm5F cells either alone or in combination. In addition, these cytokines do not significantly potentiate or attenuate the IL-1 response. This unprecedented specificity to IL-1 has been further developed as a sensitive and specific assay for IL-1 bioactivity. Quantitation by this new bioassay of human IL-1beta and IL-1 released from activated murine peritoneal macrophages showed a close correlation with the quantitation of IL-1 by enzyme immunoassay (ELISA). This new bioassay, which is specific, nonradioactive and inexpensive, represents a significant improvement over current bioassays for IL-1.

Author List

Hill JR, Corbett JA, Baldwin AC, McDaniel ML

Author

John A. Corbett PhD Chair, Professor in the Biochemistry department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Biological Assay
Culture Media
Humans
Immunoenzyme Techniques
Interleukin-1
Macrophage Activation
Mice
Nitric Oxide
Nitrites
Rats
Recombinant Proteins
Spectrophotometry
Tumor Cells, Cultured