Faculty Collaboration Database

Native low density lipoprotein. Endothelial cell recruitment of mononuclear cells. Arterioscler Thromb 1991;11(5):1175-81

Date

09/11/1991

Pubmed ID

1911704

DOI

10.1161/01.atv.11.5.1175

Scopus ID

2-s2.0-0025820739 (requires institutional sign-in at Scopus site)   52 Citations

Abstract

The effect of native low density lipoprotein (LDL) on human umbilical vein endothelial cell (EC) recruitment of mononuclear cells (Monos) was investigated. ECs were exposed to LDL at atherogenic concentrations (240 mg cholesterol [Chol]/dl) for as long as 4 days (LDL-treated ECs). LDL-treated ECs bound substantially greater amounts of freshly isolated human monocytes and U937 cells than did control ECs. The enhanced Mono binding was time and LDL concentration dependent. LDL-induced binding was reduced to control levels when cycloheximide was added together with LDL, indicating that de novo protein synthesis was required. Furthermore, this LDL effect was not a general feature of apolipoproteins, as high density lipoprotein in physiologically relevant concentrations (45 mg Chol/dl, 4 days) had no effect on EC-Mono binding. Conditioned media from LDL-treated EC cultures did not increase EC binding of Monos. In contrast, minimally modified LDL increased EC-Mono binding more than eightfold. In conclusion, LDL in concentrations associated with the premature development of atherosclerosis increased EC affinity for Monos. Such LDL-induced alterations in EC physiology likely represent a proinflammatory response and an early step in atherogenesis.

Author List

Pritchard KA Jr, Tota RR, Lin JH, Danishefsky KJ, Kurilla BA, Holland JA, Stemerman MB

Author

Kirkwood A. Pritchard PhD Professor in the Surgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Arteriosclerosis
Cell Adhesion
Cells, Cultured
Endothelium, Vascular
Humans
Lipoproteins, LDL
Monocytes
Oxidation-Reduction
Umbilical Veins