Faculty Collaboration Database

Histamine: a promoter of xanthine oxidase activity in intestinal ischemia/reperfusion. J Pediatr Surg 1990 Feb;25(2):218-22; discussion 222-3

Date

02/01/1990

Pubmed ID

1689383

DOI

10.1016/0022-3468(90)90406-y

Scopus ID

2-s2.0-0025015140 (requires institutional sign-in at Scopus site)   28 Citations

Abstract

Xanthine oxidase (XO)-derived oxygen radicals are thought to play an important role in the intestinal injury resulting from ischemia and reperfusion. In vitro data shows enhanced XO activity in the presence of histamine. Histamine is known to be released during intestinal ischemia and reperfusion. The purpose of this study was to evaluate the relationship between histamine and XO in vivo in intestinal ischemia/reperfusion injury. Using an established model of gut ischemia and reperfusion, portal venous plasma was obtained and assayed for histamine levels, XO activity, and xanthine dehydrogenase (XD) activity following injury. Intestinal ischemia for 120 minutes resulted in a 200% increase in plasma histamine levels (263.4 +/- 36.9 nmol/mL control, v 548.7 +/- 35.1 nmol/mL experimental, P less than .05). Reperfusion for 15 minutes resulted in a further increase in plasma histamine (to 658.3 +/- 33.9 nmol/mL), compared with 120 minutes of ischemia alone. No significant change in plasma XO activity resulted after simple ischemia for 120 minutes. However, XO activity doubled within 15 minutes of reperfusion of the ischemic intestine (6.37 +/- 0.53 nmol O2- per milliliter per minute v 3.12 +/- 0.25 nmol O2- per milliliter per minute, P less than .05). Reperfusion for 60 minutes resulted in the maximal observed increase in plasma XO activity (9.49 +/- 0.67 nmol O2- per milliliter per minute). Analysis of XD activity demonstrated no significant decrease compared with controls until 120 minutes of ischemia and 60 minutes of reperfusion (1.62 +/- 0.49 nmol uric acid per milliliter per minute at 60 minutes of reperfusion, versus 5.02 +/- 0.52 nmol uric acid per milliliter per minute control, P less than .05).(ABSTRACT TRUNCATED AT 250 WORDS)

Author List

Caty MG, Schmeling DJ, Friedl HP, Oldham KT, Guice KS, Till GO

MESH terms used to index this publication - Major topics in bold

Animals
Germ-Free Life
Histamine
Histamine Release
Intestines
Ischemia
Male
Rats
Rats, Inbred Strains
Reperfusion Injury
Time Factors
Xanthine Dehydrogenase
Xanthine Oxidase