Nitro-L-arginine inteferes with the cadmium reduction of nitrate/griess reaction method of measuring nitric oxide production. Eur J Clin Chem Clin Biochem 1996 Feb;34(2):133-7
Date
02/01/1996Pubmed ID
8833645DOI
10.1515/cclm.1996.34.2.133Scopus ID
2-s2.0-0030043917 (requires institutional sign-in at Scopus site) 14 CitationsAbstract
Nitro-L-arginine is used as an inhibitor of nitric oxide synthase in many biological Systems. Nitric oxide (NO) is unstable and degrades to nitrite NO(2)- and nitrate NO(3)-. The colorimetric reaction of N0(2)- with Griess reagent is commonly used to measure NO(2)-. NO(3)- may be measured as NO(2)- following reduction by cadmium or cadmium/copper. We found that bradykinin increased the formation of NO(2)- by bovine coronary endothelial cells. Nitro-L-arginine further increased the formation of NO(2)-. This increase is due to the interference of nitro-L-arginine in determination of NO(3)- by the cadmium reduction to NO(2)- and Griess reagent reaction. Incubation of nitro-L-arginine with cadmium or cadmium/copper produced a product that reacts with Griess reagent to form a compound that has an absorption spectrum identical to the product formed by NO(2)- and Griess reagent. Caution must be exercised when using the NO(2)-/NO(3)- measurement by the Griess reaction to assess inhibition of nitric oxide synthase by nitro-L-arginine.
Author List
Nithipatikom K, Pratt PF, Campbell WBAuthor
William B. Campbell PhD Professor in the Pharmacology and Toxicology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCadmium
Cattle
Endothelium, Vascular
Enzyme Inhibitors
Ethylenediamines
Free Radical Scavengers
Nitrates
Nitric Oxide
Nitric Oxide Synthase
Nitrites
Nitroarginine
Oxidation-Reduction
Spectrophotometry, Ultraviolet
Sulfanilamides