Pancreatic adenocarcinoma cell line, MDAPanc-28, with features of both acinar and ductal cells. Int J Pancreatol 1996 Feb;19(1):31-8
Date
02/01/1996Pubmed ID
8656025DOI
10.1007/BF02788373Scopus ID
2-s2.0-0029933259 (requires institutional sign-in at Scopus site) 29 CitationsAbstract
CONCLUSION: We established a new human pancreatic adenocarcinoma cell line, MDAPanc-28. Studies on this new line indicate that it expressed both acinar and ductal gene products suggesting that the patterns of gene expression in the pancreatic adenocarcinoma from which this cell line arose have features similar to those of the protodifferentiated cells hypothesized by Rutter and his colleagues for the developing pancreas (1,2).
BACKGROUND: The cell line arose from a tumor that, like most pancreatic adenocarcinomas, was ductal on the basis of its histological appearance.
METHODS: Once the cell line was established in culture, they were subjected to cytogenetic analysis and tested for their ability to grow in nude mice. RNA from the cells was analyzed by Northern blot analysis and PCR of reverse transcribed cDNA for the expression of both acinar and duct cell gene products. DNA was analyzed for the presence of mutated K-ras at codon 12.
RESULTS: The cell line expressed trypsin and ribonuclease RNA, which are considered to be acinar cell markers, and carbonic anhydrase II (CAII), which is considered to be a duct-cell markers. The histological appearance of xenografts in nude mice was similar to that of the tumor from which the cell line was established. The chromosome number varied between 46 and 60.
Author List
Frazier ML, Fernández E, de Llorens R, Brown NM, Pathak S, Cleary KR, Abbruzzese JL, Berry K, Olive M, Le Maistre A, Evans DBAuthor
Douglas B. Evans MD Chair, Professor in the Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AdenocarcinomaAged
Animals
Base Sequence
Biomarkers, Tumor
Blotting, Northern
Carbonic Anhydrases
Female
Genes, ras
Humans
Mice
Mice, Nude
Molecular Sequence Data
Pancreatic Neoplasms
Point Mutation
Polymerase Chain Reaction
RNA, Neoplasm
Ribonucleases
Transplantation, Heterologous
Trypsin
Tumor Cells, Cultured
