Faculty Collaboration Database

Endogenous vascular synthesis of B-type and C-type natriuretic peptides in the rainbow trout. J Exp Biol 2011 Aug 15;214(Pt 16):2709-17

Date

07/29/2011

Pubmed ID

21795567

Pubmed Central ID

PMC3144849

DOI

10.1242/jeb.052415

Scopus ID

2-s2.0-79961063212 (requires institutional sign-in at Scopus site)   7 Citations

Abstract

In mammals, natriuretic peptides (NPs) lower blood pressure, reduce blood volume and broadly inhibit cardiovascular remodeling. NPs are often referred to as cardiac hormones, though they also have integral roles in regulating vascular tone, endothelial remodeling and inhibiting vascular smooth muscle cell hypertrophy. Two NPs [atrial (ANP) and C-type (CNP)] have been identified as endogenous constituents in the vasculature of mammals, though such a phenomenon has not previously been described in fishes. Here we describe the endogenous production of B-type NP (BNP) and CNP in multiple blood vessels of the rainbow trout. Western blot analysis showed pro-BNP and pro-CNP production in the efferent branchial artery, celiacomesenteric artery, ventral aorta and anterior cardinal vein. The detection of pro-BNP and pro-CNP was also supported by MALDI-TOF mass spectrometry analysis of NP-enriched tissue extracts. Although vascular pro-peptide levels of BNP and CNP were quantitatively quite comparable to those found in reference tissues (the atrium for BNP and brain for CNP), mRNA levels of these NPs in the vasculature were greatly reduced as determined by quantitative PCR. When the evolutionarily conserved vascular NP (CNP) was infused into un-anesthetized trout, it reduced central venous pressure and mean circulatory filling pressure. CNP also decreased cardiac output via a reduction in preload. The presence of endogenous NP production in the trout vasculature and potent in vivo hypotensive effects further support the numerous functional similarities between teleost and mammalian NP systems.

Author List

Johnson KR, Hoagland TM, Olson KR

MESH terms used to index this publication - Major topics in bold

Animals
Base Sequence
Blood Vessels
Female
Gene Expression Regulation
Heart Function Tests
Male
Molecular Sequence Data
Natriuretic Peptide, Brain
Natriuretic Peptide, C-Type
Oncorhynchus mykiss
Organ Specificity
Proteomics
RNA, Messenger
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Vascular Capacitance