High-throughput scanning of the rat genome using interspersed repetitive sequence-PCR markers. Genomics 2000 Nov 01;69(3):287-94
Date
11/01/2000Pubmed ID
11056046DOI
10.1006/geno.2000.6352Scopus ID
2-s2.0-0034331226 (requires institutional sign-in at Scopus site) 12 CitationsAbstract
We report the establishment of a hybridization-based marker system for the rat genome based on the PCR amplification of interspersed repetitive sequences (IRS). Overall, 351 IRS markers were mapped within the rat genome. The IRS marker panel consists of 210 nonpolymorphic and 141 polymorphic markers that were screened for presence/absence polymorphism patterns in 38 different rat strains and substrains that are commonly used in biomedical research. The IRS marker panel was demonstrated to be useful for rapid genome screening in experimental rat crosses and high-throughput characterization of large-insert genomic library clones. Information on corresponding YAC clones is made available for this IRS marker set distributed over the whole rat genome. The two existing rat radiation hybrid maps were integrated by placing the IRS markers in both maps. The genetic and physical mapping data presented provide substantial information for ongoing positional cloning projects in the rat.
Author List
Gösele C, Hong L, Kreitler T, Rossmann M, Hieke B, Gross U, Kramer M, Himmelbauer H, Bihoreau MT, Kwitek-Black AE, Twigger S, Tonellato PJ, Jacob HJ, Schalkwyk LC, Lindpaintner K, Ganten D, Lehrach H, Knoblauch MAuthor
Anne E. Kwitek PhD Professor in the Physiology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCell Line
Chromosome Mapping
Chromosomes, Artificial, Yeast
Cricetinae
Genetic Markers
Genome
Interspersed Repetitive Sequences
Polymerase Chain Reaction
Rats
Rats, Inbred F344
Rats, Inbred Strains