Drosophila nonsense suppressors: functional analysis in Saccharomyces cerevisiae, Drosophila tissue culture cells and Drosophila melanogaster. Genetics 1990 Nov;126(3):625-37
Date
11/01/1990Pubmed ID
2174393Pubmed Central ID
PMC1204218DOI
10.1093/genetics/126.3.625Scopus ID
2-s2.0-0025119674 (requires institutional sign-in at Scopus site) 5 CitationsAbstract
Amber (UAG) and opal (UGA) nonsense suppressors were constructed by oligonucleotide site-directed mutagenesis of two Drosophila melanogaster leucine-tRNA genes and tested in yeast, Drosophila tissue culture cells and transformed flies. Suppression of a variety of amber and opal alleles occurs in yeast. In Drosophila tissue culture cells, the mutant tRNAs suppress hsp70:Adh (alcohol dehydrogenase) amber and opal alleles as well as an hsp70:beta-gal (beta-galactosidase) amber allele. The mutant tRNAs were also introduced into the Drosophila genome by P element-mediated transformation. No measurable suppression was seen in histochemical assays for Adhn4 (amber), AdhnB (opal), or an amber allele of beta-galactosidase. Low levels of suppression (approximately 0.1-0.5% of wild type) were detected using an hsp70:cat (chloramphenicol acetyltransferase) amber mutation. Dominant male sterility was consistently associated with the presence of the amber suppressors.
Author List
Garza D, Medhora MM, Hartl DLMESH terms used to index this publication - Major topics in bold
Alcohol DehydrogenaseAnimals
Base Sequence
Blotting, Western
Cells, Cultured
DNA Transposable Elements
Drosophila melanogaster
Molecular Sequence Data
Mutation
RNA, Transfer, Leu
Reproduction
Saccharomyces cerevisiae
Suppression, Genetic
Transformation, Genetic
