Issues with the assay of factor VIII activity in plasma and factor VIII concentrates. Thromb Haemost 2000 Dec;84(6):942-8
Date
01/12/2001Pubmed ID
11154139Scopus ID
2-s2.0-0034533788 (requires institutional sign-in at Scopus site) 33 CitationsAbstract
A review of the literature suggests that assays accurate for the determination of factor VIII in plasma samples may not necessarily retain this accuracy when used for the determination of factor VIII in high-purity factor VII concentrates such as Hemofil M. Review of assay data suggests that it is imperative to obtain maximal activation of the factor VIII in the sample with thrombin when using an assay system of isolated coagulation factors such as the two-stage assay or the various chromogenic substrate assays. Based on a combination of ease and reproducibility of performance and correlation of in vivo and in vitro measurements. it is recommended that the one-stage activated partial thromboplastin time performed with plasma from an individual with severe hemophilia A be used for the measurement of factor VIII potency. Chromogenic substrate assays can be used if care is taken to assure optimal activation of factor VIII by thrombin in the assay and the presence of sufficient factor IXa, phospholipid and calcium ions to stabilize factor VIIIa during the assay process.
Author List
Lundblad RL, Kingdon HS, Mann KG, White GCAuthor
Gilbert C. White MD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Blood Coagulation TestsChromogenic Compounds
Factor VIII
Humans
Reproducibility of Results
Sensitivity and Specificity
