Faculty Collaboration Database

The localization of the cAMP-dependent protein kinase phosphorylation site in the platelet rat protein, rap 1B. FEBS Lett 1991 Jun 03;283(2):173-6

Date

06/03/1991

Pubmed ID

1904369

DOI

10.1016/0014-5793(91)80581-m

Scopus ID

2-s2.0-0025855013 (requires institutional sign-in at Scopus site)   9 Citations

Abstract

Rap 1B is a low molecular weight G protein which is phosphorylated by cAMP-dependent protein kinase. In order to identify the site of phosphorylation by cAMP-dependent protein kinase, purified rap 1B from human platelets was phosphorylated and subjected to limited proteolysis with trypsin. Single digestion fragment containing the phosphorylation site was obtained and purified by reversed-phase HPLC. Sequence analysis of the phosphorylated digestion fragment demonstrated that the sequence of the phosphorylation site was -Lys-Lys-Ser-Ser-. This sequence is near the carboxy terminus and is adjacent to the site of membrane attachment of the protein.

Author List

Fischer TH, Collins JH, Gatling MN, White GC 2nd

Author

Gilbert C. White MD Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Amino Acid Sequence
Blood Platelets
GTP-Binding Proteins
Humans
Molecular Sequence Data
Peptide Fragments
Phosphorylation
Protein Kinases
Sequence Homology, Nucleic Acid
Trypsin
rap GTP-Binding Proteins