The localization of the cAMP-dependent protein kinase phosphorylation site in the platelet rat protein, rap 1B. FEBS Lett 1991 Jun 03;283(2):173-6
Date
06/03/1991Pubmed ID
1904369DOI
10.1016/0014-5793(91)80581-mScopus ID
2-s2.0-0025855013 (requires institutional sign-in at Scopus site) 9 CitationsAbstract
Rap 1B is a low molecular weight G protein which is phosphorylated by cAMP-dependent protein kinase. In order to identify the site of phosphorylation by cAMP-dependent protein kinase, purified rap 1B from human platelets was phosphorylated and subjected to limited proteolysis with trypsin. Single digestion fragment containing the phosphorylation site was obtained and purified by reversed-phase HPLC. Sequence analysis of the phosphorylated digestion fragment demonstrated that the sequence of the phosphorylation site was -Lys-Lys-Ser-Ser-. This sequence is near the carboxy terminus and is adjacent to the site of membrane attachment of the protein.
Author List
Fischer TH, Collins JH, Gatling MN, White GC 2ndAuthor
Gilbert C. White MD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Amino Acid SequenceBlood Platelets
GTP-Binding Proteins
Humans
Molecular Sequence Data
Peptide Fragments
Phosphorylation
Protein Kinases
Sequence Homology, Nucleic Acid
Trypsin
rap GTP-Binding Proteins