Antibody response to low-molecular-weight antigens of Aspergillus fumigatus in allergic bronchopulmonary aspergillosis. J Clin Microbiol 1989 Jun;27(6):1312-6
Date
06/01/1989Pubmed ID
2666440Pubmed Central ID
PMC267548DOI
10.1128/jcm.27.6.1312-1316.1989Scopus ID
2-s2.0-0024409608 (requires institutional sign-in at Scopus site) 36 CitationsAbstract
Sera from patients with allergic bronchopulmonary aspergillosis (ABPA) or aspergilloma and normal sera were analyzed for specific antibodies by Western (immuno-) blotting with Aspergillus fumigatus antigens transferred electrophoretically onto polyvinylidene difluoride membranes. Western blot analysis demonstrated consistent reactivity of low-molecular-weight A. fumigatus antigens against ABPA sera but not against uncomplicated aspergilloma or normal sera. None of these low-molecular-weight components had any lectin-binding activity. Sera from patients with aspergilloma, however, frequently reacted with high-molecular-weight components of A. fumigatus. The majority of these high-molecular-weight antigenic components demonstrated concanavalin A-binding activity. The low-molecular-weight bands were discernible in Western blots with sera from all ABPA patients irrespective of disease activities, such as relapse, flare, or treatment. Antibodies detected by methods such as immunodiffusion or enzyme-linked immunosorbent assays demonstrated total antibody responses to most or all antigenic components, while Western blots demonstrated the reactivities of the individual components with the specific antibodies. Western blot analysis thus provided more information for immunodiagnosis of ABPA than other methods, especially when only crude antigens were available.
Author List
Kurup VP, Greenberger PA, Fink JNMESH terms used to index this publication - Major topics in bold
Antibodies, FungalAntigens, Fungal
Aspergillosis, Allergic Bronchopulmonary
Aspergillus fumigatus
Blotting, Western
Humans
Immune Sera
Immunoglobulin E
Immunoglobulin G
Lectins
