Effects of pterostilbene on melanoma alone and in synergy with inositol hexaphosphate. Am J Surg 2009 Nov;198(5):679-84
Date
11/06/2009Pubmed ID
19887199DOI
10.1016/j.amjsurg.2009.07.014Scopus ID
2-s2.0-71749101328 (requires institutional sign-in at Scopus site) 22 CitationsAbstract
BACKGROUND: Pterostilbene and inositol-6-phosphate (IP6) have been shown to inhibit melanoma growth in vitro. However, pterostilbene's mechanism of action has not been clearly demonstrated. We aimed to further investigate the mechanism of action for pterostilbene and to determine whether combination treatment with IP6 produced synergistic growth inhibition.
METHODS: Melanoma cells were treated with increasing doses of pterostilbene, IP6, or combinations thereof. Cell viability was measured at 24 hours, 48 hours, and 72 hours using a MTT assay. Caspase activity and vascular endothelial growth factor (VEGF) production were measured using enzyme-linked immunosorbent assay (ELISA). Analysis of variance (ANOVA) and t tests were used for statistical analysis.
RESULTS: Pterostilbene inhibits melanoma growth in vitro in association with increased effector caspase activity. Combination treatment with inositol hexaphosphate produces synergistic growth inhibition, greater than either treatment alone.
CONCLUSIONS: Pterostilbene produces caspase-dependent apoptosis in melanoma cell lines. Combination treatment with IP6 produces synergistic growth inhibition. Both compounds have significant potential for a therapeutic role in the treatment of melanoma.
Author List
Schneider JG, Alosi JA, McDonald DE, McFadden DWAuthor
John G. Schneider MD Assistant Professor in the Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
CaspasesCell Survival
Colorimetry
DNA Fragmentation
Drug Screening Assays, Antitumor
Drug Therapy, Combination
Humans
Melanoma
Phytic Acid
Phytotherapy
Pterocarpus
Stilbenes
Tumor Cells, Cultured
Vascular Endothelial Growth Factor A
