The T cell activation marker CD150 can be used to identify alloantigen-activated CD4(+)25+ regulatory T cells. Cell Immunol 2004 Feb;227(2):129-39
Date
05/12/2004Pubmed ID
15135295DOI
10.1016/j.cellimm.2004.03.003Scopus ID
2-s2.0-2342634326 (requires institutional sign-in at Scopus site) 17 CitationsAbstract
We have been investigating whether alloantigen-specific CD4(+)25+ regulatory T cells can be identified for use in treating graft-versus-host disease. CD150, which is upregulated on the surface of all activated T lymphocytes, was identified as a candidate marker for alloantigen-activated CD4(+)25+ regulatory T cells by gene chip analysis. Freshly isolated CD4(+)25+ cells had only low cell-surface expression of CD150, comparable to that of CD4(+)25- T cells. Increased CD150 expression was observed on all T cells after coculture with allogeneic stimulator cells. When purified CD4(+)25+ cells were precultured with allogeneic stimulator cells, then sorted into CD150+ and CD150- subsets, allosuppressive activity was contained primarily in the CD150+ fraction. These cells also suppressed the proliferation of alloantigen-activated autologous T cells, and they could be expanded in vitro without loss of their suppressive capacity. These results suggest that CD150 can be used as a marker for the identification of purified alloantigen-activated CD4(+)25+ regulatory T cells.
Author List
Browning MB, Woodliff JE, Konkol MC, Pati NT, Ghosh S, Truitt RL, Johnson BDAuthor
Bryon D. Johnson PhD Adjunct Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Antigens, CDCD4 Antigens
Glycoproteins
Humans
Immunoglobulins
Isoantigens
Lymphocyte Activation
Lymphocyte Culture Test, Mixed
Receptors, Antigen, T-Cell
Receptors, Cell Surface
Receptors, Interleukin-2
Signaling Lymphocytic Activation Molecule Family Member 1
T-Lymphocytes
