S-Nitrosothiols: cellular formation and transport. Free Radic Biol Med 2005 Apr 01;38(7):831-8
Date
03/08/2005Pubmed ID
15749378DOI
10.1016/j.freeradbiomed.2004.12.016Scopus ID
2-s2.0-14644401128 (requires institutional sign-in at Scopus site) 237 CitationsAbstract
This review will focus on the transport and intracellular formation of S-nitrosothiols in cell culture models. The major points made in this article are: (1) S-Nitrosothiols are actively metabolized by cells. (2) S-Nitrosothiols affect cells in ways distinctly different from those of nitric oxide and can act through mechanisms that do not involve the intermediacy of nitric oxide. (3) Some S-nitrosothiols (S-nitrosocysteine, S-nitrosohomocysteine) can be taken up into cells via amino acid transport system L, whereas others (S-nitrosoglutathione, S-nitroso-N-acetylpenicillamine) are not directly transported, but require the presence of cysteine and/or cystine before the nitroso functional group is transported. (4) Proteomic detection of intracellular S-nitrosothiols is currently possible only if cells are loaded with high levels of S-nitrosothiols, and methodological advances are required in order to examine the S-nitrosated proteome after exposure of cells to physiological levels of nitric oxide.
Author List
Zhang Y, Hogg NAuthor
Neil Hogg PhD Associate Dean, Professor in the Biophysics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBiological Transport
Cells, Cultured
Humans
Rats
S-Nitrosothiols