Improved MIN6 β-cell function on self-assembled peptide amphiphile nanomatrix inscribed with extracellular matrix-derived cell adhesive ligands. Macromol Biosci 2013 Oct;13(10):1404-12
Date
08/24/2013Pubmed ID
23966265DOI
10.1002/mabi.201300155Scopus ID
2-s2.0-84885948382 (requires institutional sign-in at Scopus site) 12 CitationsAbstract
Understanding the role of the pancreatic extracellular matrix (ECM) in supporting islet survival and function drives the pursuit to create biomaterials that imitate and restore the pancreatic ECM microenvironment. To create an ECM mimic holding bioinductive cues for β-cells, self-assembled peptide amphiphiles (PAs) inscribed with four selected ECM-derived cell adhesive ligands are synthesized. After 7 days, compared to control groups cultured on biologically inert substrates, MIN6 β-cells cultured on PAs functionalized with YIGSR and RGDS cell adhesive ligands exhibit elevated insulin secretion in responses to glucose and also form β-cell clusters. These findings suggest that the self-assembled PA nanomatrix may be utilized to improve pancreatic islet transplantation for treating type 1 diabetes.
Author List
Lim DJ, Antipenko SV, Vines JB, Andukuri A, Hwang PT, Hadley NT, Rahman SM, Corbett JA, Jun HWAuthor
John A. Corbett PhD Chair, Professor in the Biochemistry department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Cell AdhesionCell Proliferation
Cell Survival
Cellular Microenvironment
Diabetes Mellitus, Type 1
Extracellular Matrix
Humans
Insulin
Insulin-Secreting Cells
Ligands
Nanostructures
Peptides
Tissue Engineering
