The costimulatory molecules CD80, CD86 and OX40L are up-regulated in Aspergillus fumigatus sensitized mice. Clin Exp Immunol 2005 Nov;142(2):242-50
Date
10/20/2005Pubmed ID
16232210Pubmed Central ID
PMC1809515DOI
10.1111/j.1365-2249.2005.02905.xScopus ID
2-s2.0-27644433870 (requires institutional sign-in at Scopus site) 14 CitationsAbstract
Aspergillus fumigatus (Af) is a fungus associated with allergic bronchopulmonary aspergillosis (ABPA) and other allergic diseases. Immune responses in these diseases are due to T and B cell responses. T cell activation requires both Af-specific engagement of the T-cell-receptor as well as interaction of antigen independent costimulatory molecules including CD28-CD80/CD86 and OX40-OX40L interactions. Since these molecules and their interactions have been suggested to have a potential involvement in the pathogenesis of ABPA, we have investigated their role in a model of experimental allergic aspergillosis. BALB/c mice were primed and sensitized with Af allergens, with or without exogenous IL-4. Results showed up-regulation of both CD86 and CD80 molecules on lung B cells from Af-sensitized mice (79% CD86+ and 24% CD80+) and Af/rIL-4-treated mice (90% CD86+ and 24% CD80+) compared to normal controls (36% and 17%, respectively). Lung macrophages in Af-sensitized mice treated or not with IL-4 showed enhanced expression of these molecules. OX40L expression was also up-regulated on lung B cells and macrophages from both Af-sensitized and Af/rIL-4 exposed mice as compared to normal controls. All Af-sensitized animals showed peripheral blood eosinophilia, enhanced total serum IgE and allergen-specific IgG1 antibodies and characteristic lung inflammation. The up-regulation of CD80, CD86 and OX40L molecules on lung B cells and macrophages from Af-allergen exposed mice suggests a major role for these molecules in the amplification and persistence of immunological and inflammatory responses in ABPA.
Author List
Barrios CS, Johnson BD, D Henderson J Jr, Fink JN, Kelly KJ, Kurup VPAuthor
Bryon D. Johnson PhD Adjunct Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AllergensAnimals
Aspergillosis, Allergic Bronchopulmonary
Aspergillus fumigatus
B7-1 Antigen
B7-2 Antigen
Cells, Cultured
Cytokines
Eosinophilia
Female
Immunoglobulin E
Immunoglobulin G
Interleukin-4
Lung
Membrane Glycoproteins
Mice
Mice, Inbred BALB C
OX40 Ligand
Spleen
Tumor Necrosis Factors
Up-Regulation